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A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris

Miguel, Sissi and Legrand, Guillaume and Duriot, Léonor and Delporte, Marianne and Menin, Barbara and Michel, Cindy and Olry, Alexandre and Chataigne, Gabrielle and Salwinski, Aleksander and Bygdell, Joakim and Vercaigne, Dominique and Wingsle, Gunnar and Hilbert, Jean Louis and Bourgaud, Frédéric and Hehn, Alain and Gagneul, David (2020). A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris. Communications biology. 3 , 673
[Research article]

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Abstract

The synthesis of 3,5-dicaffeoylquinic acid (3,5-DiCQA) has attracted the interest of many researchers for more than 30 years. Recently, enzymes belonging to the BAHD acyltransferase family were shown to mediate its synthesis, albeit with notably low efficiency. In this study, a new enzyme belonging to the GDSL lipase-like family was identified and proven to be able to transform chlorogenic acid (5-O-caffeoylquinic acid, 5-CQA, CGA) in 3,5-DiCQA with a conversion rate of more than 60%. The enzyme has been produced in different expression systems but has only been shown to be active when transiently synthesized in Nicotiana benthamiana or stably expressed in Pichia pastoris. The synthesis of the molecule could be performed in vitro but also by a bioconversion approach beginning from pure 5-CQA or from green coffee bean extract, thereby paving the road for producing it on an industrial scale. Miguel et al. identify a new enzyme belonging to the GDSL lipase-like family that is involved in the final stage of transformation of 5-CQA into 3,5-diCQA. This enzyme is able to realize an efficient transformation by over 60%, making the transformation process a valuable technological tool that can be easily transferred on an industrial scale.

Authors/Creators:Miguel, Sissi and Legrand, Guillaume and Duriot, Léonor and Delporte, Marianne and Menin, Barbara and Michel, Cindy and Olry, Alexandre and Chataigne, Gabrielle and Salwinski, Aleksander and Bygdell, Joakim and Vercaigne, Dominique and Wingsle, Gunnar and Hilbert, Jean Louis and Bourgaud, Frédéric and Hehn, Alain and Gagneul, David
Title:A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris
Series Name/Journal:Communications biology
Year of publishing :2020
Volume:3
Article number:673
Number of Pages:13
Publisher:NATURE RESEARCH
ISSN:2399-3642
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-109985
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109985
Additional ID:
Type of IDID
DOI10.1038/s42003-020-01387-1
Web of Science (WoS)000595689400003
ID Code:21279
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:19 Jan 2021 14:03
Metadata Last Modified:19 Jan 2021 14:11

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