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Molecular diagnosis and characterization of honey bee pathogens

Forsgren, Eva (2009). Molecular diagnosis and characterization of honey bee pathogens. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2009:79
ISBN 978-91-576-7426-5
[Doctoral thesis]

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Abstract

Bees are crucial for maintaining biodiversity by pollination of numerous plant species. The European honey bee, Apis mellifera, is of great importance not only for the honey they produce, but also as vital pollinators of agricultural and horticultural crops. The economical value of pollination has been estimated to be several billion dollars, and pollinator declines are a global biodiversity threat. Hence, honey bee health has great impact on the economy, food production and biodiversity worldwide. A broad spectrum of specific pathogens affect the honey bee colony including bacteria, viruses, microscopic fungi, and internal and external parasites. Some of these microorganisms and parasites are more harmful than others and infections/infestations may lead to colony collapse. Knowledge of the biology and epidemiology of these pathogens are needed for prevention of disease outbreak. The use of molecular methods has increased during recent years offering a selection of powerful tools for laboratories involved in honey bee disease diagnostics and research. Novel diagnostic techniques also allow for new approaches to honey bee pathology where specific and critical questions can be answered using modern molecular technology. This thesis focuses on molecular techniques and their application within honey bee pathology. Specific questions concerning five disease-causing agents all capable of inducing population depletion of honey bee colonies were addressed. The studied pathogens included two different bacterial species, (Melissococcus plutonius and Paenibacillus larvae) that cause serious disease in honey bee larvae, one virus (deformed wing virus, DWV) that causes deformity during pupal development and finally, two honey bee pathogenic microsporidia (Nosema ceranae and Nosema apis) that cause disease in adult bees. Resolving questions concerning prevalence and characterization of these pathogens using newly developed molecular methods has contributed to a better understanding of host-pathogen interactions and epidemiology in the honey bee system. Furthermore, the attained knowledge contributes to improved disease diagnostics and disease prevention in the beekeeping industry and expands the general field of honey bee epidemiology.

Authors/Creators:Forsgren, Eva
Title:Molecular diagnosis and characterization of honey bee pathogens
Year of publishing :2009
Volume:2009:79
Number of Pages:58
Papers/manuscripts:
NumberReferences
ALLI Forsgren E, Cassel-Lundhagen A, Imdorf A, Fries I (2005). Distribution of Melissococcus plutonius in honey bee colonies with and without symptoms of European foulbrood. Microbial Ecology 50, 369-374. II Genersch E, Forsgren E, Pentikäinen J, Ashiralieva A, Rauch S, Kilwinski J, Fries I (2006). Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation. International Journal of Systematic and Evolutionary Microbiology 56, 501-511. III Forsgren E, de Miranda R J, Isaksson M, Wei S, Fries I (2009). Deformed wing virus associated with Tropilaelaps mercedesae infesting European honey bees (Apis mellifera). Experimental and Applied Acarology 47, 87-97. IV Forsgren E, Fries I. Comparative virulence of Nosema ceranae and Nosema apis in European honey bees (Submitted manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7426-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:apis mellifera, honey bees, pathogens, pathogenicity, bacteria, viruses, varroa, pest mites, nosema, laboratory diagnosis, pcr
Keywords:Apis mellifera, Melissococcus plutonius, Paenibacillus larvae, deformed wing virus, Nosema ceranae, PCR, qPCR, rep-PCR, PFGE
URN:NBN:urn:nbn:se:slu:epsilon-3010
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3010
ID Code:2155
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: Eva Forsgren
Deposited On:10 Nov 2009 00:00
Metadata Last Modified:02 Dec 2014 10:16

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