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New insights into ethylene signalling and wood development

Love, Jonathan (2009). New insights into ethylene signalling and wood development. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2009:88
ISBN 978-91-576-7435-7
[Doctoral thesis]

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Abstract

This thesis describes experimental work concerning the biology of ethylene in the development of xylem in hybrid aspen, Populus tremula L. x tremuloides Michx. Ethylene applied to wood forming tissue has previously been shown to stimulate radial growth in trees. In dicotyledonous angiosperm trees, ethylene has also been implicated in the response to leaning stress when tension wood forms. Tension wood is characterized by a localized stimulation of cambial growth, altered patterning of cell differentiation, and the synthesis of an additional cell wall layer with altered chemistry. Strong functional evidence linking endogenous ethylene to wood formation, however, has remained elusive. Transgenic hybrid aspen trees with reduced sensitivity to ethylene were generated by ectopically expressing the Arabidopsis thaliana dominant mutant allele of the ethylene receptor ETR1 (Atetr1-1). Aspects of wood growth and development suppressed by Atetr1-1 were assayed by applying ethylene or its immediate metabolic precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), to independent transgenic lines and by comparing responses with wild-type. Remarkably, the rate of cell proliferation in tension wood of leaning trees with ectopic expression of Atetr1-1 was reduced. For the first time, this result provides conclusive evidence that ethylene is an endogenous regulator of wood formation. 1-methylcyclopropene (1-MCP), a gaseous structural analogue to ethylene which efficiently inhibits ethylene action was used as a positive control for ethylene insensitivity. Applied ACC and ethylene was observed to influence bulk density, ultrastructure, mechanical properties, and the ratio of S-type to G-type lignin in the secondary cell walls of wood. In addition, a resource describing the transcriptional expression of ETHYLENE RESPONSE FACTORS is also presented as an initial effort to elucidate downstream molecular signalling.

Authors/Creators:Love, Jonathan
Title:New insights into ethylene signalling and wood development
Year of publishing :2009
Volume:2009:88
Number of Pages:93
Papers/manuscripts:
NumberReferences
ALL1. Andersson-Gunnerås, S., Mellerowicz, E. J., Ohmiya, Y., Nilsson, P., Henrissat; B., Love, J., Moritz, T. and Sundberg, B. (2006) Biosythesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and development regulators in secondary wall biosynthesis. Plant Journal 45: 144-165 2. Love, J., Björklund, S., Vahala, J., Hertzberg, M., Kangasjärvi, J. and Sundberg, B. (2009) Ethylene is an endogenous stimulator of cell division in the cambial meristem of Populus. Proc. Natl. Acad. Sci. USA 106: 5984-5989. 3. Vahala, J., Love, J., Kumar, M., Rao, S., Björklund, S., Kangasjärvi, J. and Sundberg, B. Transcript profiling of Ethylene Response Factors in response to ethylene, 1-aminocyclopropane-1-carboxylic acid and leaning in Populus stems. Ms 4. Love, J., Rueggeberg, M., Gerber, L., Bjurhager, I., Vahala, J., Gorzás, A., Burgert, I. and Sundberg, B. Ethylene has the potential to remodel the woody cell wall of Populus. Ms
Place of Publication:Umeå
ISBN for printed version:978-91-576-7435-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:populus tremula, populus tremuloides, xylem, ethylene, arabidopsis thaliana, cambium, lignins, cellulose, growth
Keywords:wood, ethylene, cambium, lignin, cellulose, growth, ERF, ETR1
URN:NBN:urn:nbn:se:slu:epsilon-3021
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3021
ID Code:2172
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: Jonathan Love
Deposited On:20 Nov 2009 00:00
Metadata Last Modified:02 Dec 2014 10:16

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