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Seewis hantavirus in common shrew (Sorex araneus) in Sweden

Lwande, Olivia Wesula and Mohamed, Nahla and Bucht, Goran and Ahlm, Clas and Olsson, Gert and Evander, Magnus (2020). Seewis hantavirus in common shrew (Sorex araneus) in Sweden. Virology Journal. 17 , 198
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Abstract

Background Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other small mammals such as Soricomorpha (shrews, moles) and Chiroptera (bats), suggested as reservoirs for potential pandemic viruses and to play a role in the evolution of hantaviruses. It is important to study the global virome in different reservoirs, therefore our aim was to investigate whether shrews in Sweden carried any hantaviruses. Moreover, to accurately determine the host species, we developed a molecular method for identification of shrews. Method Shrews (n = 198), caught during 1998 in Sweden, were screened with a pan-hantavirus PCR using primers from a conserved region of the large genome segment. In addition to morphological typing of shrews, we developed a molecular based typing method using sequencing of the mitochondrial cytochrome C oxidase I (COI) and cytochrome B (CytB) genes. PCR amplified hantavirus and shrew fragments were sequenced and phylogenetically analysed. Results Hantavirus RNA was detected in three shrews. Sequencing identified the virus as Seewis hantavirus (SWSV), most closely related to previous isolates from Finland and Russia. All three SWSV sequences were retrieved from common shrews (Sorex araneus) sampled in Vasterbotten County, Sweden. The genetic assay for shrew identification was able to identify native Swedish shrew species, and the genetic typing of the Swedish common shrews revealed that they were most similar to common shrews from Russia. Conclusion We detected SWSV RNA in Swedish common shrew samples and developed a genetic assay for shrew identification based on the COI and CytB genes. This was the first report of presence of hantavirus in Swedish shrews.

Authors/Creators:Lwande, Olivia Wesula and Mohamed, Nahla and Bucht, Goran and Ahlm, Clas and Olsson, Gert and Evander, Magnus
Title:Seewis hantavirus in common shrew (Sorex araneus) in Sweden
Series Name/Journal:Virology Journal
Year of publishing :2020
Volume:17
Article number:198
Number of Pages:5
Publisher:BMC
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Evolutionary Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Hantavirus, Shrew, Seewis virus, Cytochrome C oxidase I gene, Cytochrome B gene, Molecular barcoding
URN:NBN:urn:nbn:se:slu:epsilon-p-110162
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110162
Additional ID:
Type of IDID
DOI10.1186/s12985-020-01477-w
Web of Science (WoS)000603568300001
ID Code:21729
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Wildlife, Fish and Environmental Studies
Deposited By: SLUpub Connector
Deposited On:25 Jan 2021 08:50
Metadata Last Modified:25 Jan 2021 08:51

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