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The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus

Abedi, Tayebeh and Castilleux, Romain and Nibbering, Pieter and Niittylä, Totte (2020). The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus. Frontiers in Plant Science. 11 , 611607
[Research article]

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Abstract

Plant cell wall associated hydroxyproline-rich glycoproteins (HRGPs) are involved in several aspects of plant growth and development, including wood formation in trees. HRGPs such as arabinogalactan-proteins (AGPs), extensins (EXTs), and proline rich proteins (PRPs) are important for the development and architecture of plant cell walls. Analysis of publicly available gene expression data revealed that many HRGP encoding genes show tight spatio-temporal expression patterns in the developing wood of Populus that are indicative of specific functions during wood formation. Similar results were obtained for the expression of glycosyl transferases putatively involved in HRGP glycosylation. In situ immunolabelling of transverse wood sections using AGP and EXT antibodies revealed the cell type specificity of different epitopes. In mature wood AGP epitopes were located in xylem ray cell walls, whereas EXT epitopes were specifically observed between neighboring xylem vessels, and on the ray cell side of the vessel walls, likely in association with pits. Molecular mass and glycan analysis of AGPs and EXTs in phloem/cambium, developing xylem, and mature xylem revealed clear differences in glycan structures and size between the tissues. Separation of AGPs by agarose gel electrophoresis and staining with beta-D-glucosyl Yariv confirmed the presence of different AGP populations in phloem/cambium and xylem. These results reveal the diverse changes in HRGP-related processes that occur during wood formation at the gene expression and HRGP glycan biosynthesis levels, and relate HRGPs and glycosylation processes to the developmental processes of wood formation.

Authors/Creators:Abedi, Tayebeh and Castilleux, Romain and Nibbering, Pieter and Niittylä, Totte
Title:The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation in Populus
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2020
Volume:11
Article number:611607
Number of Pages:24
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:hydroxyproline-rich glycoproteins, arabinogalactan-proteins, β-D-glucosyl Yariv, extensins, Populus, wood formation
URN:NBN:urn:nbn:se:slu:epsilon-p-110182
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110182
Additional ID:
Type of IDID
DOI10.3389/fpls.2020.611607
Web of Science (WoS)000602618900001
ID Code:21757
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:26 Jan 2021 13:43
Metadata Last Modified:26 Jan 2021 13:51

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