Abstract

The purpose of this study was to characterize the endogenous cellulase gene MaCel1 of Monochamus alternatus, which is an important vector of Bursaphelenchus xylophilus, a pine wood nematode, which causes pine wilt disease (PWD). In this study, MaCel1 was cloned by rapid amplification of cDNA end (RACE), and its expression analyzed by RT-qPCR (real-time quantitative PCR detecting). A total of 1778 bp of cDNA was obtained. The encoding region of this gene was 1509 bp in length, encoding a protein containing 502 amino acids with a molecular weight of 58.66 kDa, and the isoelectric point of 5.46. Sequence similarity analysis showed that the amino acids sequence of MaCel1 had high similarity with the beta-Glucosinolate of Anoplophora glabripennis and slightly lower similarity with other insect cellulase genes (GH1). The beta-D-Glucosidase activity of MaCel1 was 256.02 +/- 43.14 U/L with no beta-Glucosinolate activity. MaCel1 gene was widely expressed in the intestine of M. alternatus. The expression level of MaCel1 gene in male (3.46) and female (3.51) adults was significantly higher than that in other developmental stages, and the lowest was in pupal stage (0.15). The results will help reveal the digestive mechanism of M. alternatus and lay the foundation for controlling PWD by controlling M. alternatus.

Keywords

Monochamus alternatus; endogenous cellulase; cloning; RT-qPCR

Published in

Forests
2020, Volume: 11, number: 12, article number: 1372
Publisher: MDPI

SLU Authors

• Tigabu, Mulualem

  • Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network


UKÄ Subject classification

Forest Science


Publication identifier

DOI: https://doi.org/10.3390/f11121372

Permanent link to this page (URI)

https://res.slu.se/id/publ/110210