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Molecular Cloning and Expression Analysis of the Endogenous Cellulase Gene MaCel1 in Monochamus alternatus

Li, Yachao and Chen, Hao and Chu, Xu and Ma, Qiuyu and Liang, Guanghong and Wu, Songqing and Wang, Rong and Tigabu, Mulualem and Zhang, Feiping and Hu, Xia (2020). Molecular Cloning and Expression Analysis of the Endogenous Cellulase Gene MaCel1 in Monochamus alternatus. Forests. 11 , 1372
[Research article]

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Abstract

The purpose of this study was to characterize the endogenous cellulase gene MaCel1 of Monochamus alternatus, which is an important vector of Bursaphelenchus xylophilus, a pine wood nematode, which causes pine wilt disease (PWD). In this study, MaCel1 was cloned by rapid amplification of cDNA end (RACE), and its expression analyzed by RT-qPCR (real-time quantitative PCR detecting). A total of 1778 bp of cDNA was obtained. The encoding region of this gene was 1509 bp in length, encoding a protein containing 502 amino acids with a molecular weight of 58.66 kDa, and the isoelectric point of 5.46. Sequence similarity analysis showed that the amino acids sequence of MaCel1 had high similarity with the beta-Glucosinolate of Anoplophora glabripennis and slightly lower similarity with other insect cellulase genes (GH1). The beta-D-Glucosidase activity of MaCel1 was 256.02 +/- 43.14 U/L with no beta-Glucosinolate activity. MaCel1 gene was widely expressed in the intestine of M. alternatus. The expression level of MaCel1 gene in male (3.46) and female (3.51) adults was significantly higher than that in other developmental stages, and the lowest was in pupal stage (0.15). The results will help reveal the digestive mechanism of M. alternatus and lay the foundation for controlling PWD by controlling M. alternatus.

Authors/Creators:Li, Yachao and Chen, Hao and Chu, Xu and Ma, Qiuyu and Liang, Guanghong and Wu, Songqing and Wang, Rong and Tigabu, Mulualem and Zhang, Feiping and Hu, Xia
Title:Molecular Cloning and Expression Analysis of the Endogenous Cellulase Gene MaCel1 in Monochamus alternatus
Series Name/Journal:Forests
Year of publishing :2020
Volume:11
Article number:1372
Number of Pages:13
Publisher:MDPI
ISSN:1999-4907
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:Monochamus alternatus, endogenous cellulase, cloning, RT-qPCR
URN:NBN:urn:nbn:se:slu:epsilon-p-110210
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110210
Additional ID:
Type of IDID
DOI10.3390/f11121372
Web of Science (WoS)000601963600001
ID Code:21773
Faculty:S - Faculty of Forest Sciences
Department:(S) > Southern Swedish Forest Research Centre
Deposited By: SLUpub Connector
Deposited On:27 Jan 2021 14:43
Metadata Last Modified:27 Jan 2021 14:51

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