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Changes in lipid and carotenoid metabolism in Chlamydomonas reinhardtii during induction of CO2-concentrating mechanism: Cellular response to low CO2 stress

Abreu, Ilka and Aksmann, Anna and Bajhaiya, Amit K. and Benlloch, Reyes and Giordano, Mario and Pokora, Wojciech and Selstam, Eva and Moritz, Thomas (2020). Changes in lipid and carotenoid metabolism in Chlamydomonas reinhardtii during induction of CO2-concentrating mechanism: Cellular response to low CO2 stress. Algal Research. 52 , 102099
[Research article]

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Abstract

Photosynthetic organisms strictly depend on CO2 availability and the CO2:O-2 ratio, as both CO2/O-2 compete for catalytic site of Rubisco. Green alga Chlamydomonas reinhardtii, can overcome CO2 shortage by inducing CO2-concentrating mechanism (CCM). Cells transferred to low-CO2 are subjected to light-driven oxidative stress due to decrease in the electron sink. Response to environmental perturbations is mediated to some extent by changes in the lipid and carotenoid metabolism. We thus hypothesize that when cells are challenged with changes in CO2 availability, changes in the lipidome and carotenoids profile occur. These changes expected to be transient, when CCM is activated, CO2 limitation will be substantially ameliorated. In our experiments, cells were transferred from high (5%) to low (air equilibrium) CO2. qPCR analysis of genes related to CCM and lipid metabolism was carried out. Lipidome was analyzed both in whole cells and in isolated lipid droplets. We characterized the changes in polar lipids, fatty acids and ketocarotenoids. In general, polar lipids significantly and transiently increased in lipid droplets during CCM. Similar pattern was observed for xanthophylls, ketocarotenoids and their esters. The data supports our hypothesis about the roles of lipids and carotenoids in tackling the oxidative stress associated with acclimation to sub-saturating CO2.

Authors/Creators:Abreu, Ilka and Aksmann, Anna and Bajhaiya, Amit K. and Benlloch, Reyes and Giordano, Mario and Pokora, Wojciech and Selstam, Eva and Moritz, Thomas
Title:Changes in lipid and carotenoid metabolism in Chlamydomonas reinhardtii during induction of CO2-concentrating mechanism: Cellular response to low CO2 stress
Series Name/Journal:Algal Research
Year of publishing :2020
Volume:52
Article number:102099
Number of Pages:12
Publisher:ELSEVIER
ISSN:2211-9264
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Betaine lipids, Carotenogenesis, CCM, Chlamydomonas, Lipid droplets
URN:NBN:urn:nbn:se:slu:epsilon-p-110303
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110303
Additional ID:
Type of IDID
DOI10.1016/j.algal.2020.102099
Web of Science (WoS)000596690900015
ID Code:21800
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:01 Feb 2021 04:43
Metadata Last Modified:01 Feb 2021 06:01

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