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Large lungworms (Nematoda: Dictyocaulidae) recovered from the European bison may represent a new nematode subspecies

Pyziel, Anna M. and Laskowski, Zdzislaw and Dolka, Izabella and Kolodziej-Sobocinska, Marta and Nowakowska, Julita and Klich, Daniel and Bielecki, Wojciech and Zygowska, Marta and Moazzami, Madeleine and Anusz, Krzysztof and Höglund, Johan (2020). Large lungworms (Nematoda: Dictyocaulidae) recovered from the European bison may represent a new nematode subspecies. International Journal for Parasitology: Parasites and Wildlife. 13 , 213-220
[Research article]

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Abstract

Although the Dictyocaulus lungworm, the agent of dictyocaulosis, is one of parasitological threats to European bison, its systematic position remains unclear. The aim of the present study was to evaluate the morphological features of the lungworm and the pathological lesions it induces, and to analyse mitochondrial (mt) genetic markers for systematic and molecular epidemiological studies. The morphological findings indicate that Dictyocaulus lungworms of European bison can be distinguished from those of cattle on the basis of differences in buccal capsule wall length, total body length, and spicules length in males, all of which were significantly longer in those of European bison. Nucleotide diversity calculated from pairwise sequence alignments of partial cytochrome c oxidase subunit 1 (cox1), cytochrome B (cytB) and NADH dehydrogenase subunit 5 (nad5) of specimens from cattle and European bison varied from 1.7% for nad5, 2.1% for cytB, to 3.7% for cox1 gene. Thus, among the lungworms of European bison and cattle, nad5 and cytB were the most conserved proteins, whereas coxl was the most diverse. The mt cytB marker gene may be a suitable candidate for distinguishing between the two genotypes, as nad5 demonstrated the greatest within-genus sequence variation. The lung tissue of infected European bison manifests signs of verminous pneumonia characterized by interstitial pneumonia, bronchitis and bronchiolitis. Therefore, it appears that European bison and cattle are infected with slightly diverged, morphologically-different, genotypes of D. viviparus, indicating they belong to two separate worm populations. We propose, therefore, that the lungworm of European bison should be classified as D. viviparus subsp. bisontis.

Authors/Creators:Pyziel, Anna M. and Laskowski, Zdzislaw and Dolka, Izabella and Kolodziej-Sobocinska, Marta and Nowakowska, Julita and Klich, Daniel and Bielecki, Wojciech and Zygowska, Marta and Moazzami, Madeleine and Anusz, Krzysztof and Höglund, Johan
Title:Large lungworms (Nematoda: Dictyocaulidae) recovered from the European bison may represent a new nematode subspecies
Series Name/Journal:International Journal for Parasitology: Parasites and Wildlife
Year of publishing :2020
Volume:13
Page range:213-220
Number of Pages:8
Publisher:ELSEVIER
ISSN:2213-2244
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology
Keywords:Dictyocaulus viviparus, Bison bonasus, Morphological description, Verminous pneumonia, Mitochondrial genetic markers
URN:NBN:urn:nbn:se:slu:epsilon-p-110345
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110345
Additional ID:
Type of IDID
DOI10.1016/j.ijppaw.2020.10.002
Web of Science (WoS)000600626200028
ID Code:21807
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:01 Feb 2021 09:23
Metadata Last Modified:01 Feb 2021 09:31

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