structural studies of Phanerochaete chrysosporium Laminarinase 16A
Vasur, Jonas
(2009).
Probing promiscuity.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2009:98
ISBN 978-91-576-7445-6
[Doctoral thesis]
![]() |
PDF
25MB |
![]() |
PDF
25MB |
Abstract
The glycoside hydrolase Laminarinase 16A (Lam16A) from the white-rot fungus Phanerochaete chrysosporiumı is able to bind and cleave two different substrates in a specific manner. It has been called "non-specific", but we find that binding promiscuity and catalytic promiscuity better define this phenomenon. We have published high-resolution structures of wildtype Lam16A alone (Paper I) and in complex with oligosaccharide products from hydrolysis of laminarin and lichenan (up to 1.1 Å resolution) (Paper II) as well as structures of the catalytic nucleophile mutant Lam16A E115S in complex with laminariheptaose and alpha-laminariheptaosyl fluoride (Paper III, submitted). A beta-sandwich forms the substrate binding cleft, evenly open at both ends. The Lam16A ligand/complex structures, especially the catalytic mutants (Paper IV, manuscript), illustrate why two beta-1,3-linked glucose residues are required at the -2 and -1 subsites and why no beta-1,6-branch can be accomodated at these sites. Also, catalytic mutants enabled the trapping of substrate in the +1 subsite, showing how Lam16A can hydrolyze both beta-1,4- and beta-1,3-glycosidic bonds.
Authors/Creators: | Vasur, Jonas | ||||
---|---|---|---|---|---|
Title: | Probing promiscuity | ||||
Subtitle: | structural studies of Phanerochaete chrysosporium Laminarinase 16A | ||||
Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||
Year of publishing : | 2009 | ||||
Number: | 2009:98 | ||||
Number of Pages: | 36 | ||||
Papers/manuscripts: |
| ||||
Place of Publication: | Uppsala | ||||
ISBN for printed version: | 978-91-576-7445-6 | ||||
ISSN: | 1652-6880 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agrovoc terms: | phanerochaete chrysosporium, hydrolases, beta glucanase, glucosides, enzyme activity, ligands, hydrolysis, analytical methods | ||||
Keywords: | 1, 3(4)-beta-D-glucanase, promiscuous, enzyme, protein-ligand structure, lichenan, lichenin, laminarin, glycoside hydrolase family 16 | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-3026 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3026 | ||||
ID Code: | 2186 | ||||
Department: | (NL, NJ) > Dept. of Molecular Biology (until 131231) | ||||
Deposited By: | Jonas Vasur | ||||
Deposited On: | 25 Nov 2009 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:16 |
Repository Staff Only: item control page