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Assessment of antidiabetic potential and phytochemical profiling of Rhazya stricta root extracts

Mahmood, Rashid and Kayani, Waqas Khan and Ahmed, Tanveer and Malik, Farnaz and Hussain, Shahzad and Ashfaq, Muhammad and Ali, Hussain and Rubnawaz, Samina and Green, Brian D. and Calderwood, Danielle and Kenny, Owen and Rivera, Gerardo A. and Mirza, Bushra and Rasheed, Faiza (2020). Assessment of antidiabetic potential and phytochemical profiling of Rhazya stricta root extracts. BMC Complementary Medicine and Therapies. 20 , 293
[Research article]

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Abstract

Background: Diabetes mellitus is a chronic disease characterized by hyperglycemia that may occur due to genetic, environmental or lifestyle factors. Natural remedies have been used to treat diabetes since long and many antidiabetic compounds of varied efficacies have been isolated from medicinal plants. Rhazya stricta has been used for decades for the treatment of diabetes mellitus and associated ailments. Considering the folkloric use of R. stricta against diabetes, it was aimed to investigate the effectiveness of its root extracts against diabetes through in vitro assays and in vivo studies using animal model along with phytochemical profiling through GCMS.Methods: Various fractions of Rhazya stricta obtained through column chromatography were evaluated for a variety of assays including a-glucosidase, Dipeptidyl peptidase-IV (DPP-IV), beta-secretase and Glucagon-like peptide-1 (GLP-1) secretion studies. For the in vivo studies the alloxan-induced diabetic mice were treated with root extracts and blood glucose levels, HbA1C, and other biochemical markers along with the histological study of the liver were done. The phytochemical identification was performed using an Agilent 7890B GC coupled to a 7010 Triple Quadrupole (MS/MS) system. GraphPad Prism software version 5.01 was used for statistical analysis.Results: Majority of the extract fractions showed excellent results against diabetes by inhibiting enzymes DPP-IV (Up to 61%) and beta-secretase (Up to 83%) with IC50s 979 pg/ml and 169 mu g/ml respectively with increase in the GLP1 secretion. The results of in vivo studies indicated a marked reduction in blood glucose and HbA1c levels along with positive effects on other parameters like lipid profile, liver functions and renal functions of extract-treated mice as compared to control. The histological examination of the liver demonstrated hepatoprotective effects against diabetes led changes and various classes of phytochemicals were also identified through GCMS in different fractions.Conclusion: The results revealed strong antidiabetic activity of R. stricta root with the potential to protect body organs against diabetic changes. Moreover, a variety of phytochemicals has also been identified through GCMS that might be responsible for the antidiabetic potential of Rhazya stricta root.

Authors/Creators:Mahmood, Rashid and Kayani, Waqas Khan and Ahmed, Tanveer and Malik, Farnaz and Hussain, Shahzad and Ashfaq, Muhammad and Ali, Hussain and Rubnawaz, Samina and Green, Brian D. and Calderwood, Danielle and Kenny, Owen and Rivera, Gerardo A. and Mirza, Bushra and Rasheed, Faiza
Title:Assessment of antidiabetic potential and phytochemical profiling of Rhazya stricta root extracts
Series Name/Journal:BMC Complementary Medicine and Therapies
Year of publishing :2020
Volume:20
Article number:293
Number of Pages:17
Publisher:BMC
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Pharmacology and Toxicology
Keywords:Diabetes mellitus, beta-Secretase, DPP-IV, GLP-1 secretion, In vivo studies, Alloxan induced mice, GC-MS
URN:NBN:urn:nbn:se:slu:epsilon-p-108433
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108433
Additional ID:
Type of IDID
DOI10.1186/s12906-020-03035-x
Web of Science (WoS)000576966500003
ID Code:21959
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:03 Feb 2021 06:03
Metadata Last Modified:03 Feb 2021 06:11

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