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Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani

Brouwer, Sophie and Odilbekov, Firuz and Burra, Dharani and Lenman, Marit and Hedley, Pete E. and Grenville-Briggs Didymus, Laura and Alexandersson, Erik and Liljeroth, Erland and Andreasson, Erik (2020). Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani. Plant Molecular Biology. 104 , 1-19
[Research article]

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Abstract

Key Message Using disease bioassays and transcriptomic analysis we show that intact SA-signalling is required for potato defences against the necrotrophic fungal pathogenAlternaria solani. Early blight, caused by the necrotrophic fungusAlternaria solani,is an increasing problem in potato cultivation. Studies of the molecular components defining defence responses toA. solaniin potato are limited. Here, we investigate plant defence signalling with a focus on salicylic acid (SA) and jasmonic acid (JA) pathways in response toA. solani. Our bioassays revealed that SA is necessary to restrict pathogen growth and early blight symptom development in both potato foliage and tubers. This result is in contrast to the documented minimal role of SA in resistance ofArabidopsis thalianaagainst necrotrophic pathogens. We also present transcriptomic analysis with 36 arrays ofA. solaniinoculated SA-deficient, JA-insensitive, and wild type plant lines. A greater number of genes are differentially expressed in the SA-deficient mutant plant line compared to the wild type and JA- insensitive line. In wild type plants, genes encoding metal ion transporters, such as copper, iron and zinc transporters were upregulated and transferase-encoding genes, for example UDP-glucoronosyltransferase and Serine-glyoxylate transferase, were downregulated. The SA-deficient plants show upregulation of genes enriched in GO terms related to oxidoreductase activity, respiratory chain and other mitochondrial-related processes.Pathogenesis-relatedgenes, such as genes encoding chitinases and PR1, are upregulated in both the SA-deficient and wild type plants, but not in the JA-insensitive mutants. The combination of our bioassays and the transcriptomic analysis indicate that intact SA signalling, and not JA signalling, is required for potato defences against the necrotrophic pathogenA. solani.

Authors/Creators:Brouwer, Sophie and Odilbekov, Firuz and Burra, Dharani and Lenman, Marit and Hedley, Pete E. and Grenville-Briggs Didymus, Laura and Alexandersson, Erik and Liljeroth, Erland and Andreasson, Erik
Title:Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani
Series Name/Journal:Plant Molecular Biology
Year of publishing :2020
Volume:104
Page range:1-19
Number of Pages:19
Publisher:SPRINGER
ISSN:0167-4412
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Early blight, Alternaria solani, SA, JA, NahG, Coi1, Potato, Solanum tuberosum
URN:NBN:urn:nbn:se:slu:epsilon-p-106881
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106881
Additional ID:
Type of IDID
DOI10.1007/s11103-020-01019-6
Web of Science (WoS)000542775800001
ID Code:21967
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:03 Feb 2021 08:23
Metadata Last Modified:03 Feb 2021 08:31

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