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Transcriptional responses in developing lesions of European common ash (Fraxinus excelsior) reveal genes responding to infection by Hymenoscyphus fraxineus

Sahraei, Shadi Eshghi and Cleary, Michelle and Stenlid, Jan and Brandström Durling, Mikael and Elfstrand, Malin (2020). Transcriptional responses in developing lesions of European common ash (Fraxinus excelsior) reveal genes responding to infection by Hymenoscyphus fraxineus. BMC Plant Biology. 20 , 455
[Journal article]

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Abstract

BackgroundWith the expanding ash dieback epidemic that has spread across the European continent, an improved functional understanding of the disease development in afflicted hosts is needed. The study investigated whether differences in necrosis extension between common ash (Fraxinus excelsior) trees with different levels of susceptibility to the fungus Hymenoscyphus fraxineus are associated with, and can be explained by, the differences in gene expression patterns. We inoculated seemingly healthy branches of each of two resistant and susceptible ash genotypes with H. fraxineus grown in a common garden.ResultsTen months after the inoculation, the length of necrosis on the resistant genotypes were shorter than on the susceptible genotypes. RNA sequencing of bark samples collected at the border of necrotic lesions and from healthy tissues distal to the lesion revealed relatively limited differences in gene expression patterns between susceptible and resistant genotypes. At the necrosis front, only 138 transcripts were differentially expressed between the genotype categories while 1082 were differentially expressed in distal, non-symptomatic tissues. Among these differentially expressed genes, several genes in the mevalonate (MVA) and iridoid pathways were found to be co-regulated, possibly indicating increased fluxes through these pathways in response to H. fraxineus.Comparison of transcriptional responses of symptomatic and non-symptomatic ash in a controlled greenhouse experiment revealed a relatively small set of genes that were differentially and concordantly expressed in both studies. This gene-set included the rate-limiting enzyme in the MVA pathway and a number of transcription factors. Furthermore, several of the concordantly expressed candidate genes show significant similarity to genes encoding players in the abscisic acid- or Jasmonate-signalling pathways.ConclusionsA set of candidate genes, concordantly expressed between field and greenhouse experiments, was identified. The candidates are associated with hormone signalling and specialized metabolite biosynthesis pathways indicating the involvement of these pathways in the response of the host to infection by H. fraxineus.

Authors/Creators:Sahraei, Shadi Eshghi and Cleary, Michelle and Stenlid, Jan and Brandström Durling, Mikael and Elfstrand, Malin
Title:Transcriptional responses in developing lesions of European common ash (Fraxinus excelsior) reveal genes responding to infection by Hymenoscyphus fraxineus
Series Name/Journal:BMC Plant Biology
Year of publishing :2020
Volume:20
Article number:455
Number of Pages:11
Publisher:BMC
ISSN:1471-2229
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Ash dieback, Fraxinus excelsior, HMGR1 (3-hydroxy-3-methylglutaryl-coenzyme a reductase 1), ATAF1, Resistance, Necrotic lesion
URN:NBN:urn:nbn:se:slu:epsilon-p-108418
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108418
Additional ID:
Type of IDID
DOI10.1186/s12870-020-02656-1
Web of Science (WoS)000578284600001
ID Code:21977
Faculty:S - Faculty of Forest Sciences
Department:(S) > Southern Swedish Forest Research Centre
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:03 Feb 2021 10:43
Metadata Last Modified:03 Feb 2021 10:51

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