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Understanding of Molecular Mechanisms and Improvement of Adventitious Root Formation in Apple

Smolka, Anders (2010). Understanding of Molecular Mechanisms and Improvement of Adventitious Root Formation in Apple. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:8
ISBN 978-91-576-7485-2
[Doctoral thesis]

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Abstract

Adventitious root formation is essential for vegetative propagation, which is the main method for propagation of many horticultural crops, especially woody species. For propagation of apple rootstocks, adventitious root formation is often a limiting step. The molecular mechanisms underlying adventitious root formation are still largely unknown and need to be extensively investigated. In this thesis approaches towards an increased understating of adventitious root formation were made using both apple and the model plant Arabidopsis. For Arabidopsis, a protocol for efficient rooting of hypocotyls and stem segments using two pulses of auxin induction was developed. This protocol was used for studying the expression of a number of rooting related genes during adventitious root formation in both stems and hypocotyls, and for anatomical studies of the timing of the rooting process. The transcript levels of GH3-3, LBD16, LBD29, LRP1 and ARF17 were clearly affected by the auxin treatment, and some of these genes displayed considerable differences between stem segments and hypocotyls. In apple, the function of the rooting related gene ARRO-1 was investigated through down-regulation of the gene using RNAi. The results indicate a possible role for ARRO-1 in promoting adventitious root initiation or regulating hormone homeostasis. The effect of apple rootstocks transformed with the Agrobacterium rolB gene on non-transgenic scions was also investigated, regarding tree growth, flowering and fruit quality. The results showed that the transgenic rootstock had no clear effect on fruit quality but greatly affected vegetative growth. No translocation of transgene mRNA or DNA from rootstock to scion could be detected. Attempts were also made to produce ROLB specific antibodies to investigate if the ROLB protein is transported from the transgenic rootstock into the non-transgenic scion which could be of concern for the consumer.

Authors/Creators:Smolka, Anders
Title:Understanding of Molecular Mechanisms and Improvement of Adventitious Root Formation in Apple
Year of publishing :2010
Volume:2010:8
Number of Pages:41
Papers/manuscripts:
NumberReferences
ALLI Margareta Welander, Anders Smolka, Thomas Geier, Annelie Ahlman, Anna Holefors and Li-Hua Zhu (2009). Origin, Timing and Gene Expression of Adventitious Rooting in Arabidopsis Hypocotyls and Stems (manuscript). II Anders Smolka, Margareta Welander, Peter Olsson, Anna Holefors and Li-Hua Zhu (2009). Involvement of the ARRO-1 Gene in Adventitious Root Formation in Apple. Plant Science 177(6), 710-715. III Anders Smolka, Margareta Welander, Peter Olsson and Li-Hua Zhu (2009). Production of Recombinant Protein and Antibodies for ROLB Protein Analysis (manuscript). IV Anders Smolka, Xue-Yuan Li, Catrin Heikelt, Margareta Welander and Li-Hua Zhu (2009). Effects of Ttransgenic Rootstocks on Growth and Development of Non-Transgenic Scion Cultivars in Apple. Accepted for publication in Transgenic Research.
Place of Publication:Alnarp
ISBN for printed version:978-91-576-7485-2
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
Subjects:Not in use, please see Agris categories
Agrovoc terms:apples, adventitious roots, molecular biology, genes, genetic engineering, rootstocks
Keywords:Apple, adventitious root formation, Arabidopsis thaliana, ARRO-1, rolB, rooting related genes
URN:NBN:urn:nbn:se:slu:epsilon-3039
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3039
ID Code:2215
Department:(LTJ, LTV) > Plant Breeding and Biotechnology (until 121231)
Deposited By: Anders Smolka
Deposited On:25 Jan 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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