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Functional characterization of the AGL1 aegerolysin in the mycoparasitic fungusTrichoderma atroviridereveals a role in conidiation and antagonism

Dubey, Mukesh and Jensen, Dan Funck and Karlsson, Magnus (2021). Functional characterization of the AGL1 aegerolysin in the mycoparasitic fungusTrichoderma atroviridereveals a role in conidiation and antagonism. Molecular Genetics and Genomics. 296 , 131-140
[Research article]

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Abstract

Aegerolysins are small secreted pore-forming proteins that are found in both prokaryotes and eukaryotes. The role of aegerolysins in sporulation, fruit body formation, and in lysis of cellular membrane is suggested in fungi. The aim of the present study was to characterize the biological function of the aegerolysin geneagl1in the mycoparasitic fungusTrichoderma atroviride, used for biological control of plant diseases. Gene expression analysis showed higher expression ofagl1during conidiation and during growth in medium supplemented with cell wall material from the plant pathogenic fungusRhizoctonia solanias the sole carbon source. Expression ofagl1was supressed under iron-limiting condition, whileagl1transcript was not detected duringT.atrovirideinteractions with the prey fungiBotrytis cinereaorR.solani. Phenotypic analysis ofagl1deletion strains (Delta agl1) showed reduced conidiation compared toT.atroviridewild type, thus suggesting the involvement of AGL1 in conidiation. Furthermore, the Delta agl1strains display reduced antagonism towardsB. cinereaandR.solanibased on a secretion assay, although no difference was detected during direct interactions. These data demonstrate the role of AGL1 in conidiation and antagonism in the mycoparasitic fungusT.atroviride.

Authors/Creators:Dubey, Mukesh and Jensen, Dan Funck and Karlsson, Magnus
Title:Functional characterization of the AGL1 aegerolysin in the mycoparasitic fungusTrichoderma atroviridereveals a role in conidiation and antagonism
Series Name/Journal:Molecular Genetics and Genomics
Year of publishing :2021
Volume:296
Page range:131-140
Number of Pages:10
Publisher:SPRINGER HEIDELBERG
ISSN:1617-4615
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
Keywords:Aegerolysin, Antagonism, Haemolysin, Mycoparasitism, Pore-forming proteins, Trichoderma
URN:NBN:urn:nbn:se:slu:epsilon-p-108410
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108410
Additional ID:
Type of IDID
DOI10.1007/s00438-020-01732-3
Web of Science (WoS)000577220300001
ID Code:22152
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:09 Feb 2021 07:49
Metadata Last Modified:09 Feb 2021 07:51

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