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Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress

Liu, Shujing and De Jonge, Jennifer and Trejo-Arellano, Minerva S. and Santos-González, Juan and Köhler, Claudia and Hennig, Lars (2021). Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress. New Phytologist. 229 , 2238-2250
[Journal article]

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Abstract

Heat-stressed Arabidopsis plants release heterochromatin-associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to heat stress.We generated and analyzed RNA and bisulfite-sequencing data of wild-type and h1 mutant seedlings before and after heat stress.Loss of H1 caused activation of pericentromeric Gypsy elements upon heat treatment, despite these elements remaining highly methylated. By contrast, nonpericentromeric Copia elements became activated concomitantly with loss of DNA methylation. The same Copia elements became activated in heat-treated chromomethylase 2 (cmt2) mutants, indicating that H1 represses Copia elements through maintaining DNA methylation under heat.We discovered that H1 is required for TE repression in response to heat stress, but its functional role differs depending on TE location. Strikingly, H1-deficient plants treated with the DNA methyltransferase inhibitor zebularine were highly tolerant to heat stress, suggesting that both H1 and DNA methylation redundantly suppress the plant response to heat stress.

Authors/Creators:Liu, Shujing and De Jonge, Jennifer and Trejo-Arellano, Minerva S. and Santos-González, Juan and Köhler, Claudia and Hennig, Lars
Title:Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
Series Name/Journal:New Phytologist
Year of publishing :2021
Volume:229
Page range:2238-2250
Number of Pages:13
Publisher:WILEY
ISSN:0028-646X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Arabidopsis thaliana, CMT2, DNA methylation, H1, heat stress, transposable element
URN:NBN:urn:nbn:se:slu:epsilon-p-109340
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109340
Additional ID:
Type of IDID
DOI10.1111/nph.17018
Web of Science (WoS)000591172200001
ID Code:22157
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:09 Feb 2021 07:55
Metadata Last Modified:09 Feb 2021 08:01

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