Home About Browse Search
Svenska


A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera- than Coleoptera-targeted Bacillus thuringiensis strains

Tudoran, Amelia and Nordlander, Göran and Karlberg, Anna and Puentes, Adriana (2021). A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera- than Coleoptera-targeted Bacillus thuringiensis strains. Pest Management Science. 77 , 1303-1315
[Research article]

[img] PDF
774kB

Abstract

BACKGROUND The pine weevil (Hylobius abietis) is a major forest regeneration pest causing high levels of seedling mortality and economic losses. Current management relies on silviculture, stem coatings and insecticides. Here we evaluated for the first time the effects of Bacillus thuringiensis (Bt) strains on H. abietis adults: two producing the Coleoptera-targeted toxins Cry3Aa (Bt tenebrionis NB-176) and Cry8Da (Bt galleriae SDS-502), and one producing the Diptera-targeted Cry10A (Bt israelensis AM65-52). Choice and nonchoice assays using individual and mixtures of Bt formulations, containing these strains respectively, were conducted.RESULTS We found that Bt had toxic and lethal effects on H. abietis, but effects varied with strain and formulation concentration. The Diptera-targeted Bt israelensis had the most negative effects on weevil weight, feeding and mortality (70-82% feeding reduction, 65-82% greater mortality than control), whereas the effect was lower for the Coleoptera-specific Bt tenebrionis (38-42%; 37-42%) and Bt galleriae (11-30%; 15-32%). Reduced weevil feeding was observed after 3 days, and the highest mortality occurred 7-14 days following Bt exposure. However, we found no synergistic toxic effects, and no formulation combination was better than Bt israelensis alone at reducing consumption and survival. Also, pine weevils were not deterred by Bt, feeding equally on Bt-treated and non-Bt treated food.CONCLUSION There is potential to develop forest pest management measures against H. abietis that include Bt, but only the Diptera-targeted Bt israelensis would provide effective seedling protection. Its Diptera-specificity may need reconsideration, and evaluation of other Bt strains/toxins against H. abietis would be of interest.

Authors/Creators:Tudoran, Amelia and Nordlander, Göran and Karlberg, Anna and Puentes, Adriana
Title:A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera- than Coleoptera-targeted Bacillus thuringiensis strains
Series Name/Journal:Pest Management Science
Year of publishing :2021
Volume:77
Page range:1303-1315
Number of Pages:13
Publisher:JOHN WILEY AND SONS LTD
ISSN:1526-498X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:biopesticides, forest pest, forest regeneration, insecticidal toxins, pest management, plant protection
URN:NBN:urn:nbn:se:slu:epsilon-p-109024
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109024
Additional ID:
Type of IDID
DOI10.1002/ps.6144
ID Code:22166
Faculty:S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:09 Feb 2021 08:23
Metadata Last Modified:09 Feb 2021 08:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits