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A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation

Zhao, Xue and Andersson, Mariette (2021). A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation. Carbohydrate Polymers. 255 , 117503
[Journal article]

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Abstract

To determine the internal structure of barley starch without amylopectin isolation, whole starch was hydrolyzed using beta-amylase to remove the linear amylose and obtain beta-limit dextrins (beta-LDs). The beta-LDs were treated with extensive a-amylase to prepare alpha-limit dextrins (alpha-LDs), and the alpha-LDs were further hydrolyzed with beta-amylase into building blocks. The chain-length distribution of beta-LD and building block composition were analyzed by sizeexclusion chromatography and anion-exchange chromatography. The internal structure of the barley whole starches had similar pattern to barley amylopectins analyzed by conventional methods. The starch of barley amol-mutated varieties contained more short internal B-chains and less long internal B-chains than that of other varieties. The starch from amol-mutated varieties had more large building blocks than that from waxy varieties. The simplified method presented in this study can effectively characterize starch internal structure that relates to physicochemical properties of starch, although some details of amylopectin structure are not assessable.

Authors/Creators:Zhao, Xue and Andersson, Mariette
Title:A simplified method of determining the internal structure of amylopectin from barley starch without amylopectin isolation
Series Name/Journal:Carbohydrate Polymers
Year of publishing :2021
Volume:255
Article number:117503
Number of Pages:7
Publisher:ELSEVIER SCI LTD
ISSN:0144-8617
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Polymer Chemistry
Keywords:Barley starch, Amylopectin, Building block structure, alpha-Amylase, beta-Amylase, Chromatography
URN:NBN:urn:nbn:se:slu:epsilon-p-110508
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110508
Additional ID:
Type of IDID
DOI10.1016/j.carbpol.2020.117503
Web of Science (WoS)000608727300004
ID Code:22186
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:12 Feb 2021 09:40
Metadata Last Modified:12 Feb 2021 09:41

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