Home About Browse Search
Svenska


Identification, Antifungal Susceptibility and Phylogenetic Comparison of Fungi in Poultry Environment in Nigeria

Mgbeahuruike, A.C. and Aliyoo, K. and Karlsson, Magnus and Chah, K.F. (2020). Identification, Antifungal Susceptibility and Phylogenetic Comparison of Fungi in Poultry Environment in Nigeria. International Journal of Poultry Science. 19 , 548-556
[Journal article]

[img] PDF
612kB

Abstract

Objective: This study was conducted to investigate the fungal community structure in Nigerian poultry environments. Materials and Methods: In ten (layer and broiler) farms, samples were collected from drinkers, doors, feeders, floors, poles, roofs, walls and window nets. Fungal isolation was done on Sabouraud dextrose agar (SDA) followed by identification using morphological and microscopic features. The fungal identities were confirmed by sequencing the internal transcribed spacer region, followed by phylogenetic analysis. Antifungal susceptibility testing was done using nystatin (100 μg mL–1), fluconazole (25 μg mL–1) and voriconazole (1 μg mL–1). Results: A total of 244 fungi were identified in all the locations. In the layers farm, 112 fungal isolates were identified, while 132 isolates were identified in the broiler farm. In all the poultry farms, Aspergillus and Candida species had the highest occurrence of 32.4 and 24.6%, respectively while other fungi (Dematiaceous, Rhizopus, Penicillium, Mucor and Rhodotorula) had 43% occurrence. For the locations, poles and window nets had the highest isolation frequency of 15.2% each. The roofs, feeders and floors had 14.3 and 13.1%, while other locations had 27% isolation rate. Phylogenetic comparison of the isolates showed that closely related fungi from different countries formed separate clades. Candida species were sensitive to the three antifungal agents with the zone of inhibition diameter ranging from 19.08-25.36 mm. All the Aspergillus species were resistant to fluconazole but were sensitive to nystatin and voriconazole. Conclusion: Different fungi were identified in this study and they were all susceptible to nystatin antifungal agent.

Authors/Creators:Mgbeahuruike, A.C. and Aliyoo, K. and Karlsson, Magnus and Chah, K.F.
Title:Identification, Antifungal Susceptibility and Phylogenetic Comparison of Fungi in Poultry Environment in Nigeria
Series Name/Journal:International Journal of Poultry Science
Year of publishing :2020
Volume:19
Page range:548-556
Number of Pages:9
ISSN:1682-8356
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
URN:NBN:urn:nbn:se:slu:epsilon-p-110472
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110472
Additional ID:
Type of IDID
DOI10.3923/ijps.2020.548.556
ID Code:22198
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:12 Feb 2021 11:22
Metadata Last Modified:12 Feb 2021 11:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits