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Heterobasidion - conifer pathosystem

heterologous array analysis and transcriptional shift from saprotrophic to necrotrophic growth

Lundén, Karl (2010). Heterobasidion - conifer pathosystem. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:19
ISBN 978-91-576-7496-8
[Doctoral thesis]

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Abstract

In this thesis the Heterobasidion – conifer pathosystem is discussed in a symbiosis context. Heterobasidion annosum (Fr.)Bref. s.l. is a species complex with closely related species with partly overlapping host range. There are three European Heterobasidion species, H. annosum, H. abietinum and H. parviporum. In the first study it was shown that cDNA arrays printed for one species can be used to study gene expression in the other species. H. annosum can grow both as a saprotroph on dead wood or kill its conifer host as a necrotroph. This possibility to switch nutritional mode has impact on forest management as H. annosum can prevail in old wood for decades until infecting the next generation of trees. Gene expression patterns during the transition from saprotrophic to necrotrophic growth were studied in a nutrient limited microcosm system with dead and living Pinus sylvestris seedlings connected by a common mycelium. These results were compared with gene expression patterns of H. annosum, Phanerochaete chrysosporium (saprotroph) and Paxillus involutus (mutualist) growing in nutrient rich systems. In the nutrient rich comparison a higher correlation was found, than between the saprotrophic and necrotrophic growth of H. annosum where no differentially expressed genes were identified. However differences were found when the genes were annotated into functional categories by KOG groups. This suggests that differences between the two growth modes might depend on the magnitude of gene expression rather than distinct qualitative differences. The specificity of two mycorrhiza-associated Pinus genes (similar to Clavata 1 and MtN21) in comparison to known auxin-induced and defence genes through early signalling and ECM development with and without the auxin transport inhibitor TIBA was further investigated. The Clv-1-like gene seems to be associated with lateral root formation since expression was detected in root primordia during lateral root formation and in mycorrhizal roots.

Authors/Creators:Lundén, Karl
Title:Heterobasidion - conifer pathosystem
Subtitle:heterologous array analysis and transcriptional shift from saprotrophic to necrotrophic growth
Year of publishing :2010
Volume:2010:19
Number of Pages:57
Papers/manuscripts:
NumberReferences
ALLI. K. Lundén, M. Eklund, R. Finlay, J. Stenlid, F.O. Asiegbu (2008). Heterologous array analysis in Heterobasidion: Hybridisation of cDNA arrays with probe from mycelium of S, P or F-types Journal of Microbiological Methods 75, 219–224 II. K. Lundén, G. Li, M. Elfstrand, R. Finlay, J. Stenlid, F. O. Asiegbu. Analysis of gene expression during transition from saprotrophic to necrotrophic growth stage in Heterobasidion annosum. (Manuscript). III. G. Heller, K. Lundén, R. D. Finlay, F. O. Asiegbu, M. Elfstrand. Response of Pinus sylvestris to pathogenic, saprotrophic or symbiotic fungi: analysis of novel Clavata1-like and Nodulin 21-like genes. (Manuscript)
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7496-8
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:heterobasidion annosum, conifers, symbiosis, saprotroph, growth, genes, gene expression, pinus sylvestris, auxins, mycorrhizae
Keywords:Heterobasidion annosum, symbiosis, nutritional mode, saprotroph, Clv-l-like, gene expression, Pinus sylvestris, array, auxin, mycorrhiza
URN:NBN:urn:nbn:se:slu:epsilon-3048
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3048
ID Code:2222
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: Malin Elfstrand
Deposited On:17 Feb 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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