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Odor perception in three Coleoptera: molecule, receptor & neuron

Bengtsson, Jonas M. (2010). Odor perception in three Coleoptera: molecule, receptor & neuron. Diss. (sammanfattning/summary) Alnarp : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:14
ISBN 978-91-576-7491-3
[Doctoral thesis]

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Abstract

In this thesis, the sense of olfaction and its links to behavior was studied in the cetoniid chafers Pachnoda interrupta and P. marginata, and the bark beetle Ips typographus. P. interrupta is a pest on sorghum in Ethiopia, and I. typographus is a spruce pest in the palearctic, and an aim was to uncover new control methods. In I. typographus, the olfactory receptors (ORs) that determine response specificity in olfactory receptor neurons (ORNs) were investigated. Phylogenetic analysis showed that I. typographus and Tribolium castaneum ORs form a Coleoptera-specific subgroup. At the next level of the olfactory system, ORN response to food odorants was compared between P. interrupta and P. marginata. Both are opportunistic polyphages on fruits and flowers, but are present in disparate habitats. P. interrupta is found in savannah, and P. marginata in tropical Africa. The two species showed a high degree of overlap in their ORN arrays, indicating that a similar sensory strategy for food search is viable in both habitats. Field trapping with compounds eliciting strong ORN response identified a powerful attractant for P. interrupta, 2,3-butane diol. In a P. interrupta study, coupled gas chromatographic-electroantennographic detection identified antennally active compounds from sorghum, Sorghum bicolor, and another food host, Abutilon figarianum. Field tests indicated that single compounds (e.g. methyl salicylate) were more important than mixtures in attraction to these two hosts. Field studies of mating and aggregation in P. interrupta showed that unmated females were attractive to males, and to both sexes when combined with food. Female-specific compounds were identified by mass spectrometric comparison of male and female extracts. Field tests of these established that phenylacetaldehyde was highly attractive to both sexes, implying that it is part of the P. interrupta pheromone. These findings create novel possibilities for control. Phenylacetaldehyde and attractive food compounds could be used for mass trapping of P. interrupta. The identified I. typographus ORs make a search for pheromone and repellent receptors possible. Compounds that hyper-excite these receptors could be used for mass trapping or disruption of host search.

Authors/Creators:Bengtsson, Jonas M.
Title:Odor perception in three Coleoptera: molecule, receptor & neuron
Year of publishing :2010
Volume:2010:14
Number of Pages:46
Papers/manuscripts:
NumberReferences
ALLI Jonas M. Bengtsson, Martin N. Andersson, Ewald Grosse-Wilde, Marcus C. Stensmyr, Ylva Hillbur, Fredrik Schlyter, Bill S. Hansson. A Transcriptome Shortcut for Identification of Olfactory Receptor Genes in the Spruce Bark Beetle, Ips typographus (manuscript). II Jonas M. Bengtsson, Hamida Khbaish, Yitbarek Wolde-Hawariat, Andreas Reinecke, Merid Negash, Emiru Seyoum, Bill S. Hansson, Ylva Hillbur, Mattias C. Larsson. Conserved Response to Food Compounds in Olfactory Receptor Neurons of two Scarab Beetles, Pachnoda interrupta and P. marginata (manuscript). III Jonas M. Bengtsson, Yitbarek Wolde-Hawariat, Hamida Khbaish, Merid Negash, Bekele Jembere, Emiru Seyoum, Bill S. Hansson, Mattias C. Larsson, Ylva Hillbur (2009). Field Attractants for Pachnoda interrupta Selected by Means of GC-EAD and Single Sensillum Screening. Journal of Chemical Ecology 35(9), 1063-1076. IV Jonas M. Bengtsson, Satya Prabhakar Chinta, Yitbarek Wolde-Hawariat, Merid Negash, Emiru Seyoum, Bill S. Hansson, Fredrik Schlyter, Stefan Schulz, Ylva Hillbur. Pheromone-based Mating and Aggregation in the Sorghum Chafer, Pachnoda interrupta (Accepted). Paper III is reproduced with the permission of the publisher.
Place of Publication:Alnarp
ISBN for printed version:978-91-576-7491-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H10 Pests of plants
Subjects:Not in use, please see Agris categories
Agrovoc terms:coleoptera, olfaction, pheromones, monitoring, trapping
Keywords:Pachnoda interrupta, Pachnoda marginata, Ips typographus, olfaction, electrophysiology, olfactory receptor neurons, olfactory receptor proteins, pheromone, control, mass trapping, monitoring
URN:NBN:urn:nbn:se:slu:epsilon-3047
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3047
ID Code:2228
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: Jonas Bengtsson
Deposited On:11 Feb 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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