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Inflammatory response in equine joints

studies on proinflammatory cytokines in diseased joints and chondrocyte cultures

Ley, Cecilia (2010). Inflammatory response in equine joints. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2010:16
ISBN 978-91-576-7493-7
[Doctoral thesis]

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Proinflammatory cytokines mediate inflammatory responses as well as regulate tissue metabolism. Thus, they may provide a link between inflammation and other pathologic findings seen in equine joint disease. The aims of this thesis were to gain a deeper understanding of the development of chondral pathology in equine osteoarthritis (OA) by obtaining increased knowledge of inflammatory processes in the joint, and to investigate proinflammatory cytokines as markers of joint pathology. Measurements of interleukin (IL)-6 and tumour necrosis factor (TNF) activities in synovial fluid from clinical cases of carpal joint disease revealed increased concentrations of bioactive IL-6 in joints with osteochondral fragments (OCF). Immunohistochemical staining for IL-6 and high mobility group box protein (HMGB)-1 in tissues from diseased carpal and fetlock joints identified both OCF and synovial membranes as cellular sources of IL-6 and extracellular/cytoplasmic HMBG-1. Histological assessment of synovial membrane biopsies from immunostained joints showed increased grade of synovitis in diseased compared to healthy joints. The effects of IL-6, IL-1β and HMGB-1 on chondrocyte metabolism was studied in vitro, and focused on gene expression analyses. A catabolic response to IL-1β was detected, whereas the response to IL-6 and HMGB-1 was varied but indicated promotion of cartilage formation. Analyses of the ultrastructural immunolocalisation of HMGB-1 in IL-1β and IL-6 treated pellets, showed a trend for decreased nuclear/cytoplasmic ratio, and increased extracellular matrix density of HMGB-1 after IL-6 treatment. Theses studies gained insight to presence of TNF, IL-6 and HMGB-1 in equine joints, and indicated an increased inflammatory response in joints with OCF. The difference in metabolic response of chondrocytes to IL-1β versus IL-6 or HMGB-1 in vitro, and differences in HMGB-1 translocation after IL-1β and IL-6 treatment suggest that the outcome of cartilage pathology in equine joint disease may relate to the individual contributions of cytokine activity and cytokine interactions.

Authors/Creators:Ley, Cecilia
Title:Inflammatory response in equine joints
Subtitle:studies on proinflammatory cytokines in diseased joints and chondrocyte cultures
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2010
Number of Pages:89
ALLI. Ley, C., Ekman, S., Elmén, A., Nilsson, G. and Eloranta, ML. (2007). Interleukin-6 and tumour necrosis factor in synovial fluid from horses with carpal joint pathology. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine 54, 346-351. II. Ley, C., Ekman, S., Ronéus, B. and Eloranta, ML. (2009). Interleukin and high mobility group box protein-1 in synovial membranes and osteochondral fragments in equine osteoarthritis. Research in Veterinary Science 86, 490-497. III. Ley, C., Svala, E., Nilton, A., Lindahl, A., Eloranta, ML., Ekman, S. and Skiöldebrand, E. Effects of high mobility group box protein-1,interleukin-1β and -6 on cartilage matrix metabolism in three dimensional equine chondrocyte cultures (manuscript). IV. Ley, C., Svala, E., Skiöldebrand, E., Blomén, E., Hultenby, K., Eloranta,ML. and Ekman, S. Gene expression and immunolocalisation of high mobility group box protein (HMGB)-1 in interleukin (IL)-1β and -6 stimulated three dimensional chondrocyte cultures (manuscript). Papers I-II are reproduced with the kind permission of the publishers.
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7493-7
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:horses, cytokines, joint diseases
Keywords:cytokines, HMGB-1, horse, IL-1, IL-6, osteoarthritis, osteochondral fragmentation, synovitis, TNF
Permanent URL:
ID Code:2259
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Cecilia Ley
Deposited On:26 Mar 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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