Home About Browse Search
Svenska


A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine

Vortmann, Marina and Stumpf, Anna K. and Sgobba, Elvira and Dirks-Hofmeister, Mareike E. and Krehenbrink, Martin and Wendisch, Volker F. and Philipp, Bodo and Moerschbacher, Bruno M. (2021). A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine. Applied Microbiology and Biotechnology. 105 , 1547-1561
[Journal article]

[img] PDF
1MB

Abstract

Chitin is an abundant waste product from shrimp and mushroom industries and as such, an appropriate secondary feedstock for biotechnological processes. However, chitin is a crystalline substrate embedded in complex biological matrices, and, therefore, difficult to utilize, requiring an equally complex chitinolytic machinery. Following a bottom-up approach, we here describe the step-wise development of a mutualistic, non-competitive consortium in which a lysine-auxotrophic Escherichia coli substrate converter cleaves the chitin monomer N-acetylglucosamine (GlcNAc) into glucosamine (GlcN) and acetate, but uses only acetate while leaving GlcN for growth of the lysine-secreting Corynebacterium glutamicum producer strain. We first engineered the substrate converter strain for growth on acetate but not GlcN, and the producer strain for growth on GlcN but not acetate. Growth of the two strains in co-culture in the presence of a mixture of GlcN and acetate was stabilized through lysine cross-feeding. Addition of recombinant chitinase to cleave chitin into GlcNAc(2), chitin deacetylase to convert GlcNAc(2) into GlcN(2) and acetate, and glucosaminidase to cleave GlcN(2) into GlcN supported growth of the two strains in co-culture in the presence of colloidal chitin as sole carbon source. Substrate converter strains secreting a chitinase or a beta-1,4-glucosaminidase degraded chitin to GlcNAc(2) or GlcN(2) to GlcN, respectively, but required glucose for growth. In contrast, by cleaving GlcNAc into GlcN and acetate, a chitin deacetylase-expressing substrate converter enabled growth of the producer strain in co-culture with GlcNAc as sole carbon source, providing proof-of-principle for a fully integrated co-culture for the biotechnological utilization of chitin.

Authors/Creators:Vortmann, Marina and Stumpf, Anna K. and Sgobba, Elvira and Dirks-Hofmeister, Mareike E. and Krehenbrink, Martin and Wendisch, Volker F. and Philipp, Bodo and Moerschbacher, Bruno M.
Title:A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine
Series Name/Journal:Applied Microbiology and Biotechnology
Year of publishing :2021
Volume:105
Page range:1547-1561
Number of Pages:15
Publisher:SPRINGER
ISSN:0175-7598
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Microbial consortia, Chitin, N-acetylglucosamine, Escherichia coli, Corynebacterium glutamicum, Cross-feeding
URN:NBN:urn:nbn:se:slu:epsilon-p-110727
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110727
Additional ID:
Type of IDID
DOI10.1007/s00253-021-11112-5
Web of Science (WoS)000613172600003
ID Code:22636
Deposited By: SLUpub Connector
Deposited On:22 Feb 2021 11:03
Metadata Last Modified:22 Feb 2021 11:11

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits