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CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

Bünder, Anne and Sundman, Ola and Mahboubi, Amir and Persson, Staffan and Mansfield, Shawn D. and Ruggeberg, Markus and Niittylä, Totte (2020). CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen. Plant Journal. 103 , 1858-1868
[Journal article]

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Abstract

Cellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load-bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated the role of CSI1 in hybrid aspen (Populus tremula x Populus tremuloides) by characterizing transgenic lines with significantly reducedCSI1transcript abundance. Reduction in leaves (50-80%) caused leaf twisting and misshaped pavement cells, while reduction (70-90%) in developing xylem led to impaired mechanical wood properties evident as a decrease in the elastic modulus and rupture. X-ray diffraction measurements indicate that microfibril angle was not impacted by the alteredCSI1abundance in developing wood fibres. Instead, the augmented wood phenotype of the transgenic trees was associated with a reduced cellulose degree of polymerization. These findings establish a function for CSI1 in wood mechanics and in defining leaf cell shape. Furthermore, the results imply that the microfibril angle in wood is defined by CSI1 independent mechanism(s).

Authors/Creators:Bünder, Anne and Sundman, Ola and Mahboubi, Amir and Persson, Staffan and Mansfield, Shawn D. and Ruggeberg, Markus and Niittylä, Totte
Title:CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen
Series Name/Journal:Plant Journal
Year of publishing :2020
Volume:103
Page range:1858-1868
Number of Pages:11
Publisher:WILEY
ISSN:0960-7412
Language:English
Additional Information:Correction in: The Plant Journal, 2020, Volume: 104 Issue 2, pp 559-559, DOI 10.1111/tpj.15014
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Wood Science
Keywords:aspen, Populus, cell wall, wood mechanics, cellulose, transgenic trees, cellulose interacting 1, CSI1, pavement cell
URN:NBN:urn:nbn:se:slu:epsilon-p-106990
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106990
Additional ID:
Type of IDID
DOI10.1111/tpj.14873
Web of Science (WoS)000546711100001
ID Code:22640
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:22 Feb 2021 15:03
Metadata Last Modified:22 Feb 2021 15:11

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