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Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce

Chaudhary, Rajiv and Lundén, Karl and Dalman, Kerstin and Dubey, Mukesh and Nemesio Gorriz, Miguel and Karlsson, Bo and Stenlid, Jan and Elfstrand, Malin (2020). Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce. Scientific Reports. 10 , 12711
[Research article]

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Abstract

The Heterobasidion annosum s.l species complex comprises the most damaging forest pathogens to Norway spruce. We revisited previously identified Quantitative Trait Loci (QTLs) related to Heterobasidion-resistance in Norway spruce to identify candidate genes associated with these QTLs. We identified 329 candidate genes associated with the resistance QTLs using a gene-based composite map for Pinaceae. To evaluate the transcriptional responses of these candidate genes to H. parviporum, we inoculated Norway spruce plants and sequenced the transcriptome of the interaction at 3 and 7 days post inoculation. Out of 298 expressed candidate genes 124 were differentially expressed between inoculation and wounding control treatment. Interestingly, PaNAC04 and two of its paralogs in the subgroup III-3 of the NAC family transcription factors were found to be associated with one of the QTLs and was also highly induced in response to H. parviporum. These genes are possibly involved in the regulation of biosynthesis of flavonoid compounds. Furthermore, several of the differentially expressed candidate genes were associated with the phenylpropanoid pathway including a phenylalanine ammonia-lyase, a cinnamoyl-CoA reductase, a caffeoyl-CoA O-methyltransferase and a PgMYB11-like transcription factor gene. Combining transcriptome and genetic linkage analyses can help identifying candidate genes for functional studies and molecular breeding in non-model species.

Authors/Creators:Chaudhary, Rajiv and Lundén, Karl and Dalman, Kerstin and Dubey, Mukesh and Nemesio Gorriz, Miguel and Karlsson, Bo and Stenlid, Jan and Elfstrand, Malin
Title:Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce
Series Name/Journal:Scientific Reports
Year of publishing :2020
Volume:10
Article number:12711
Number of Pages:14
ISSN:2045-2322
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
URN:NBN:urn:nbn:se:slu:epsilon-p-109412
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109412
Additional ID:
Type of IDID
DOI10.1038/s41598-020-69386-0
Web of Science (WoS)000556385700024
ID Code:22654
Faculty:S - Faculty of Forest Sciences
NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:24 Feb 2021 11:43
Metadata Last Modified:01 Mar 2021 02:47

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