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Multi-Parental Populations Suitable for Identifying Sources of Resistance to Powdery Mildew in Winter Wheat

Vagndorf Nordestgaard, Nana and Thach, Tine and Sarup, Pernille and Rodriguez-Algaba, Julian and Reitan Andersen, Jeppe and Hovmoller, Mogens Stovring and Jahoor, Ahmed and Jorgensen, Lise Nistrup and Orabi, Jihad (2021). Multi-Parental Populations Suitable for Identifying Sources of Resistance to Powdery Mildew in Winter Wheat. Frontiers in Plant Science. 11 , 570863
[Research article]

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Abstract

Wheat (Triticum aestivum L.) is one of the world's staple food crops and one of the most devastating foliar diseases attacking wheat is powdery mildew (PM). In Denmark only a few specific fungicides are available for controlling PM and the use of resistant cultivars is often recommended. In this study, two Chinese wheat landraces and two synthetic hexaploid wheat lines were used as donors for creating four multi-parental populations with a total of 717 individual lines to identify new PM resistance genetic variants. These lines and the nine parental lines (including the elite cultivars used to create the populations) were genotyped using a 20 K Illumina SNP chip, which resulted in 8,902 segregating single nucleotide polymorphisms for assessment of the population structure and whole genome association study. The largest genetic difference among the lines was between the donors and the elite cultivars, the second largest genetic difference was between the different donors; a difference that was also reflected in differences between the four multi-parental populations. The 726 lines were phenotyped for PM resistance in 2017 and 2018. A high PM disease pressure was observed in both seasons, with severities ranging from 0 to >50%. Whole genome association studies for genetic variation in PM resistance in the populations revealed significant markers mapped to either chromosome 2A, B, or D in each of the four populations. However, linkage disequilibrium between these putative quantitative trait loci (QTL) were all above 0.80, probably representing a single QTL. A combined analysis of all the populations confirmed this result and the most associated marker explained 42% of the variation in PM resistance. This study gives both knowledge about the resistance as well as molecular tools and plant material that can be utilised in marker-assisted selection. Additionally, the four populations produced in this study are highly suitable for association studies of other traits than PM resistance.

Authors/Creators:Vagndorf Nordestgaard, Nana and Thach, Tine and Sarup, Pernille and Rodriguez-Algaba, Julian and Reitan Andersen, Jeppe and Hovmoller, Mogens Stovring and Jahoor, Ahmed and Jorgensen, Lise Nistrup and Orabi, Jihad
Title:Multi-Parental Populations Suitable for Identifying Sources of Resistance to Powdery Mildew in Winter Wheat
Series Name/Journal:Frontiers in Plant Science
Year of publishing :2021
Volume:11
Article number:570863
Number of Pages:11
Publisher:FRONTIERS MEDIA SA
ISSN:1664-462X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:GWAS, Fungal disease, powdery mildew, wheat, multi-parental population
URN:NBN:urn:nbn:se:slu:epsilon-p-110778
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110778
Additional ID:
Type of IDID
DOI10.3389/fpls.2020.570863
Web of Science (WoS)000614398700001
ID Code:22663
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:25 Feb 2021 13:23
Metadata Last Modified:25 Feb 2021 13:31

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