Svanberg, Sofie and Li, Zhiqiang and Öhlund, Pontus and Roy, Ananya and Åbrink, Magnus
(2020).
Mast Cells Limit Ear Swelling Independently of the Chymase Mouse Mast Cell Protease 4 in an MC903-Induced Atopic Dermatitis-Like Mouse Model.
International Journal of Molecular Sciences. 21
, 6311
[Research article]
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Abstract
Atopic dermatitis (AD) is a complex, often lifelong allergic disease with severe pruritus affecting around 10% of both humans and dogs. To investigate the role of mast cells (MCs) and MC-specific proteases on the immunopathogenesis of AD, a vitamin D-3-analog (MC903) was used to induce clinical AD-like symptoms in c-kit-dependent MC-deficient Wsh(-/-)and the MC protease-deficient mMCP-4(-/-), mMCP-6(-/-), and CPA3(-/-)mouse strains. MC903-treatment on the ear lobe increased clinical scores and ear-thickening, along with increased MC and granulocyte infiltration and activity, as well as increased levels of interleukin 33 (IL-33) locally and thymic stromal lymphopoietin (TSLP) both locally and systemically. The MC-deficient Wsh(-/-)mice showed significantly increased clinical score and ear thickening albeit having lower ear tissue levels of IL-33 and TSLP as well as lower serum levels of TSLP as compared to the WT mice. In contrast, although having significantly increased IL-33 ear tissue levels the chymase-deficient mMCP-4(-/-)mice showed similar clinical score, ear thickening, and TSLP levels in ear tissue and serum as the WT mice, whereas mMCP-6 and CPA3 -deficient mice showed a slightly reduced ear thickening and granulocyte infiltration. Our results suggest that MCs promote and control the level of MC903-induced AD-like inflammation.
Authors/Creators: | Svanberg, Sofie and Li, Zhiqiang and Öhlund, Pontus and Roy, Ananya and Åbrink, Magnus | ||||||
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Title: | Mast Cells Limit Ear Swelling Independently of the Chymase Mouse Mast Cell Protease 4 in an MC903-Induced Atopic Dermatitis-Like Mouse Model | ||||||
Series Name/Journal: | International Journal of Molecular Sciences | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 21 | ||||||
Article number: | 6311 | ||||||
Number of Pages: | 18 | ||||||
Publisher: | MDPI | ||||||
ISSN: | 1661-6596 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology | ||||||
Keywords: | mouse mast cell chymase 4 (mMCP-4), knockout, MC903, atopic dermatitis, IL-33, thymic stromal lymphopoietin | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-108157 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-108157 | ||||||
Additional ID: |
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ID Code: | 22676 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Department of Biomedical Science and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 01 Mar 2021 08:43 | ||||||
Metadata Last Modified: | 01 Mar 2021 08:51 |
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