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Molecular signatures of local adaptation to light in Norway spruce

Ranade, Sonali and Garcia Gil, Rosario (2021). Molecular signatures of local adaptation to light in Norway spruce. Planta. 253 , 53
[Research article]

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Abstract

Main conclusionTranscriptomic and exome capture analysis reveal an adaptive cline for shade tolerance in Norway spruce. Genes involved in the lignin pathway and immunity seem to play a potential role in contributing towards local adaptation to light.AbstractThe study of natural variation is an efficient method to elucidate how plants adapt to local climatic conditions, a key process for the evolution of a species. Norway spruce is a shade-tolerant conifer in which the requirement of far-red light for growth increases latitudinally northwards. The objective of the study is to characterize the genetic control of local adaptation to light enriched in far-red in Norway spruce, motivated by a latitudinal gradient for the Red:Far-red (R:FR) ratio to which Norway spruce has been proven to be genetically adapted. We have established the genomic signatures of local adaptation by conducting transcriptomic (total RNA-sequencing) and genomic analyses (exome capture), for the identification of genes differentially regulated along the cline. RNA-sequencing revealed 274 differentially expressed genes in response to SHADE (low R:FR light), between the southern and northern natural populations in Sweden. Exome capture included analysis of a uniquely large data set (1654 trees) that revealed missense variations in coding regions of nine differentially expressed candidate genes, which followed a latitudinal cline in allele and genotype frequencies. These genes included five transcription factors involved in vital processes like bud-set/bud-flush, lignin pathway, and cold acclimation and other genes that take part in cell-wall remodeling, secondary cell-wall thickening, response to starvation, and immunity. Based on these results, we suggest that the northern populations might not only be able to adjust their growing season in response to low R:FR light, but they may also be better adapted towards disease resistance by up-regulation of the lignin pathway that is linked to immunity. This forms a concrete basis for local adaptation to light quality in Norway spruce, one of the most economically important conifer tree species in Sweden.

Authors/Creators:Ranade, Sonali and Garcia Gil, Rosario
Title:Molecular signatures of local adaptation to light in Norway spruce
Series Name/Journal:Planta
Year of publishing :2021
Volume:253
Article number:53
Number of Pages:18
Publisher:SPRINGER
ISSN:0032-0935
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:Conifers, Exome capture, Immunity, Latitudinal cline, Lignin, Local adaptation, Missense variation, Norway spruce, R:FR ratio, Response to shade, SNP
URN:NBN:urn:nbn:se:slu:epsilon-p-110951
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110951
Additional ID:
Type of IDID
DOI10.1007/s00425-020-03517-9
Web of Science (WoS)000616148400003
ID Code:22720
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:09 Mar 2021 10:03
Metadata Last Modified:09 Mar 2021 10:11

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