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Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector

Tzelepis, Georgios and Dölfors, Fredrik and Holmquist, Louise and Dixelius, Christina (2021). Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector. Biochemical and Biophysical Research Communications. 544 , 86-90
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Abstract

The fungal species Rhizoctonia solani belongs to the Basidiomycota division and is a ubiquitous soil-borne pathogen. It is the main agent of the damping-off disease in seedlings and causes the root and crown rot disease in sugar beets. Plant pathogens deploy small secreted proteins, called effectors, to manipulate plant immunity in order to infect the host. Here, a gene (RsCRP1) encoded a putative effector cysteine-rich protein was cloned, expressed in Cercospora beticola and used for virulence assays. The RsCRP1 gene was highly induced upon the early-infection stage of sugar beet seedlings and disease was promoted. Confocal microscopy demonstrated localization to the chloroplasts and mitochondria upon transient expression of RsCRP1 in leaves of Nicotiana benthamiana. Further, this effector was unable to induce necrosis or to suppress hypersensitive response induced by the Avr4/Cf4 complex in N. benthamiana. Overall, these data indicate that RsCRP1 is a novel effector targeting distinct plant cell organelles in order to facilitate a successful infection at the early stages of the disease development. (C) 2021 The Authors. Published by Elsevier Inc.

Authors/Creators:Tzelepis, Georgios and Dölfors, Fredrik and Holmquist, Louise and Dixelius, Christina
Title:Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector
Series Name/Journal:Biochemical and Biophysical Research Communications
Year of publishing :2021
Volume:544
Page range:86-90
Number of Pages:5
Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:0006-291X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:Basidiomycete, Chloroplast, Effector, Mitochondria, Soilborne pathogen
URN:NBN:urn:nbn:se:slu:epsilon-p-110995
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110995
Additional ID:
Type of IDID
DOI10.1016/j.bbrc.2021.01.019
Web of Science (WoS)000618854200013
ID Code:22740
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology

(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:11 Mar 2021 13:43
Metadata Last Modified:11 Mar 2021 13:51

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