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Evaluation of fasting plasma insulin and proxy measurements to assess insulin sensitivity in horses

Truelsen Lindåse, Sanna and Nostell, Katarina and Bergsten, Peter and Forslund, Anders and Bröjer, Johan (2021). Evaluation of fasting plasma insulin and proxy measurements to assess insulin sensitivity in horses. BMC Veterinary Research. 17 , 78
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Abstract

BackgroundProxies are mathematical calculations based on fasting glucose and/or insulin concentrations developed to allow prediction of insulin sensitivity (IS) and beta -cell response. These proxies have not been evaluated in horses with insulin dysregulation. The first objective of this study was to evaluate how fasting insulin (FI) and proxies for IS (1/Insulin, reciprocal of the square root of insulin (RISQI) and the quantitative insulin sensitivity check index (QUICKI)) and beta -cell response (the modified insulin-to-glucose ratio (MIRG) and the homeostatic model assessment of beta -cell function (HOMA-beta)) were correlated to measures of IS (M index) using the euglycemic hyperinsulinemic clamp (EHC) in horses with insulin resistance (IR) and normal IS. A second objective was to evaluate the repeatability of FI and proxies in horses based on sampling on consecutive days. The last objective was to investigate the most appropriate cut-off value for the proxies and FI.ResultsThirty-four horses were categorized as IR and 26 as IS based on the M index. The proxies and FI had coefficients of variation (CVs)<= 25.3% and very good reliability (intraclass correlation coefficients >= 0.89). All proxies and FI were good predictors of the M index (r=0.76-0.85; P<0.001). The proxies for IS had a positive linear relationship with the M index whereas proxies for -cell response and FI had an inverse relationship with the M index. Cut-off values to distinguish horses with IR from horses with normal IS based on the M index were established for all proxies and FI using receiver operating characteristic curves, with sensitivity between 79% and 91% and specificity between 85% and 96%. The cut-off values to predict IR were <0.32 (RISQI), < 0.33 (QUICKI) and >9.5 mu IU/mL for FI.ConclusionsAll proxies and FI provided repeatable estimates of horses' IS. However, there is no advantage of using proxies instead of FI to estimate IR in the horse. Due to the heteroscedasticity of the data, proxies and FI in general are more suitable for epidemiological studies and larger clinical studies than as a diagnostic tool for measurement of IR in individual horses.

Authors/Creators:Truelsen Lindåse, Sanna and Nostell, Katarina and Bergsten, Peter and Forslund, Anders and Bröjer, Johan
Title:Evaluation of fasting plasma insulin and proxy measurements to assess insulin sensitivity in horses
Series Name/Journal:BMC Veterinary Research
Year of publishing :2021
Volume:17
Article number:78
Number of Pages:10
Publisher:BMC
ISSN:1746-6148
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Endocrine, Equine metabolic syndrome, Beta-cell response, Insulin dysregulation, Insulin resistance
URN:NBN:urn:nbn:se:slu:epsilon-p-111006
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111006
Additional ID:
Type of IDID
DOI10.1186/s12917-021-02781-5
Web of Science (WoS)000620254800001
ID Code:22749
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:11 Mar 2021 17:03
Metadata Last Modified:11 Mar 2021 17:11

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