Kendall, Anna and Nystrom, S. and Ekman, Stina and Hulten, Lm and Lindahl, A. and Hansson, E. and Skiöldebrand, Eva
(2021).
Nerve growth factor in the equine joint.
Veterinary Journal. 267
, 105579
[Research article]
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Abstract
Nerve growth factor (NGF) is a neurotrophin with many functions. In humans, it is involved in inflammation, nerve growth, apoptosis and pain signalling. Increased concentrations of NGF in synovial fluid has been shown in humans and dogs with osteoarthritis. Despite osteoarthritis being a common problem in horses, no studies have previously been published on NGF in the equine joint. The aim of this study was to quantify NGF in equine synovial fluid from healthy joints, acutely inflamed septic joints and joints with structural changes associated with osteoarthritis. A secondary aim was to identify the localisation of NGF and its two receptors, TrkA and p75(NTR) in healthy and osteoarthritic articular cartilage. NGF concentrations in synovial fluid from osteoarthritic joints (n = 27), septic joints (n = 9) and healthy joints (n = 16) were determined by ELISA. In addition, articular cartilage from osteoarthritic and healthy joints was examined for NGF, TrkA and P75(NTR) using immunohistochemistry staining. NGF was present in equine synovial fluid and articular cartilage. Compared to synovial fluid from healthy joints, NGF concentration was higher in synovial fluid from joints with structural osteoarthritic changes (P = 0.032) or acute septic inflammation (P = 0.006). In articular cartilage with severe osteoarthritic changes, there was more abundant positive immunohistochemistry staining for NGF and its receptors than in normal articular cartilage. Further studies should focus on identifying precursor forms of NGF, and on receptor expression and downstream signalling of TrkA and P75(NTR) in health and disease. (C) 2020 The Author(s). Published by Elsevier Ltd.
Authors/Creators: | Kendall, Anna and Nystrom, S. and Ekman, Stina and Hulten, Lm and Lindahl, A. and Hansson, E. and Skiöldebrand, Eva | ||||||
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Title: | Nerve growth factor in the equine joint | ||||||
Series Name/Journal: | Veterinary Journal | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 267 | ||||||
Article number: | 105579 | ||||||
Number of Pages: | 6 | ||||||
Publisher: | ELSEVIER SCI LTD | ||||||
ISSN: | 1090-0233 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology | ||||||
Keywords: | Horse, Joint, Nerve, Growth factor, Osteoarthritis | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-110279 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110279 | ||||||
Additional ID: |
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ID Code: | 22797 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Department of Biomedical Science and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 18 Mar 2021 15:43 | ||||||
Metadata Last Modified: | 18 Mar 2021 15:51 |
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