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Stable bull fertility protein markers in seminal plasma

Willforss, J. and Morrell, Jane and Resjö, Svante and Hallap, T. and Padrik, P. and Siino, V and De Koning, Dirk-Jan and Andreasson, Erik and Levander, F. and Humblot, Patrice (2021). Stable bull fertility protein markers in seminal plasma. Journal of Proteomics. 236 , 104135
[Research article]

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Abstract

Bull fertility is an important trait in breeding as the semen of one bull can, potentially, be used to perform thousands of inseminations. The high number of inseminations needed to obtain reliable measures from NonReturn Rates to oestrus creates difficulties in assessing fertility accurately. Improving molecular knowledge of seminal properties may provide ways to facilitate selection of bulls with good semen quality. In this study, liquid chromatography mass spectrometry (LC-MS/MS) was used to analyze the protein content from the seminal plasma of 20 bulls with Non-Return Rates between 35 and 60%, sampled across three seasons. Overall, 1343 proteins were identified and proteins with consistent correlation to fertility across multiple seasons found. From these, nine protein groups had a significant Pearson correlation (p < 0.1) with fertility in all three seasons and 34 protein groups had a similar correlation in at least two seasons. Among notable proteins showing a high and consistent correlation across seasons were Osteopontin, a lipase (LIPA) and N-acetylglucosamine-1 phospho-transferase subunit gamma. Three proteins were combined in a multiple linear regression to predict fertility (r = 0.81). These sets of proteins represent potential markers, which could be used by the breeding industry to phenotype bull fertility.Significance: The ability of bull spermatozoa to fertilize oocytes is crucial for breeding efficiency. However, the reliability of this trait from field measures is relatively low and the prediction of fertility given by conventional methods to evaluate sperm quality is currently not very accurate. In this work, we identify sets of proteins in bull seminal plasma from repeated samples collected at different times of the year that correlate to fertility in a consistent way. We combined these individual proteins to build a molecular signature predictive of fertility. This study provides an overview of proteins linked to fertility in seminal plasma, thereby increasing knowledge of the bull seminal plasma proteome. Protein signatures from the latter, potentially related to fertility, may be of use to predict fertility for individual bulls.

Authors/Creators:Willforss, J. and Morrell, Jane and Resjö, Svante and Hallap, T. and Padrik, P. and Siino, V and De Koning, Dirk-Jan and Andreasson, Erik and Levander, F. and Humblot, Patrice
Title:Stable bull fertility protein markers in seminal plasma
Series Name/Journal:Journal of Proteomics
Year of publishing :2021
Volume:236
Article number:104135
Number of Pages:13
Publisher:ELSEVIER
ISSN:1874-3919
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Bos taurus, Seminal plasma, Fertility, Proteomics, Seasonal variation
URN:NBN:urn:nbn:se:slu:epsilon-p-111096
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111096
Additional ID:
Type of IDID
DOI10.1016/j.jprot.2021.104135
Web of Science (WoS)000621737000006
ID Code:22803
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(VH) > Dept. of Clinical Sciences
(LTJ, LTV) > Department of Plant Protection Biology
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:19 Mar 2021 11:04
Metadata Last Modified:03 Nov 2021 12:08

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