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Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds

Halvarsson, Peter and Höglund, Johan (2021). Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds. Parasites and Vectors. 14 , 114
[Research article]

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Abstract

Background: A novel way to study the species composition and diversity of nematode parasites in livestock is to perform deep sequencing on composite samples containing a mixture of different species. Herein we describe for the first time the nematode community structures (nemabiomes) inhabiting Swedish sheep and how these are/were affected by host age and recent anthelmintic treatments.Methods: A total of 158 fecal samples were collected (n = 35 in 2007 and n = 123 in 2013-2016) and cultured from groups of sheep on 61 commercial farms in the south-central part of the country where most animals are grazed. Among the samples, 2 x 44 (56%) were paired collections from the same groups pre- and post-treatment with anthelmintics such as macrocyclic lactones, benzimidazoles or levamisole. Samples were analyzed for their nemabiome using the PacBio platform followed by bioinformatic sequence analysis with SCATA. Species richness and diversity were calculated and analyzed in R.Results: Nematode ITS2 sequences were found in all larval culture samples except two, even though the fecal egg counts were below the McMaster threshold in 20 samples. Sequencing yielded, on average, 1008 sequences per sample. In total, 16 operational taxonomical units (OTU), all with >= 98 % identity to sequences in the NCBI database, were recognized. The OTUs found represented nematode species of which ten are commonly associated with sheep. Multiple species were identified in all pre-anthelmintic treatment larval culture samples. No effects on nematode diversity were found in relation to host age. On the other hand, recent anthelmintic treatment lowered species richness, especially after use of ivermectin and albendazole. Interestingly, despite zero egg counts after use of levamisole, these samples still contained nematode DNA and especially H. contortus.Conclusions: Our findings provide evidence that nemabiome analysis combined with diversity index analysis provides an objective methodology in the study of the efficacy of anthelmintic treatment as both high and low abundant species were detected.

Authors/Creators:Halvarsson, Peter and Höglund, Johan
Title:Sheep nemabiome diversity and its response to anthelmintic treatment in Swedish sheep herds
Series Name/Journal:Parasites and Vectors
Year of publishing :2021
Volume:14
Article number:114
Number of Pages:12
Publisher:BMC
ISSN:1756-3305
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:PacBio, Gastrointestinal nematode, Livestock, Anthelmintics, Biodiversity, Diversity index
URN:NBN:urn:nbn:se:slu:epsilon-p-111118
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111118
Additional ID:
Type of IDID
DOI10.1186/s13071-021-04602-y
Web of Science (WoS)000621057200002
ID Code:22806
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:22 Mar 2021 07:03
Metadata Last Modified:22 Mar 2021 07:11

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