Ben Amira, Maroua and Faize, Mohamed and Karlsson, Magnus and Dubey, Mukesh and Frac, Magdalena and Panek, Jacek and Fumanal, Boris and Gousset-Dupont, Aurelie and Julien, Jean-Louis and Chaar, Hatem and Auguin, Daniel and Mom, Robin and Label, Philippe and Venisse, Jean-Stephane
(2021).
Fungal X-Intrinsic Protein Aquaporin from Trichoderma atroviride: Structural and Functional Considerations.
Biomolecules. 11
, 338
[Research article]
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Abstract
The major intrinsic protein (MIP) superfamily is a key part of the fungal transmembrane transport network. It facilitates the transport of water and low molecular weight solutes across biomembranes. The fungal uncharacterized X-Intrinsic Protein (XIP) subfamily includes the full protein diversity of MIP. Their biological functions still remain fully hypothetical. The aim of this study is still to deepen the diversity and the structure of the XIP subfamily in light of the MIP counterparts-the aquaporins (AQPs) and aquaglyceroporins (AQGPs)-and to describe for the first time their function in the development, biomass accumulation, and mycoparasitic aptitudes of the fungal bioagent Trichoderma atroviride. The fungus-XIP Glade, with one member (TriatXIP), is one of the three clades of MIPs that make up the diversity of T. atroviride MIPs, along with the AQPs (three members) and the AQGPs (three members). TriatXIP resembles those of strict aquaporins, predicting water diffusion and possibly other small polar solutes due to particularly wider ar/R constriction with a Lysine substitution at the LE2 position. The XIP loss of function in Delta TriatXIP mutants slightly delays biomass accumulation but does not impact mycoparasitic activities. Delta TriatMIP forms colonies similar to wild type; however, the hyphae are slightly thinner and colonies produce rare chlamydospores in PDA and specific media, most of which are relatively small and exhibit abnormal morphologies. To better understand the molecular causes of these deviant phenotypes, a wide-metabolic survey of the ATriatXIPs demonstrates that the delayed growth kinetic, correlated to a decrease in respiration rate, is caused by perturbations in the pentose phosphate pathway. Furthermore, the null expression of the XIP gene strongly impacts the expression of four expressed MIP-encoding genes of T. atroviride, a plausible compensating effect which safeguards the physiological integrity and life cycle of the fungus. This paper offers an overview of the fungal XIP family in the biocontrol agent T. atroviride which will be useful for further functional analysis of this particular MIP subfamily in vegetative growth and the environmental stress response in fungi. Ultimately, these findings have implications for the ecophysiology of Trichoderma spp. in natural, agronomic, and industrial systems.
Authors/Creators: | Ben Amira, Maroua and Faize, Mohamed and Karlsson, Magnus and Dubey, Mukesh and Frac, Magdalena and Panek, Jacek and Fumanal, Boris and Gousset-Dupont, Aurelie and Julien, Jean-Louis and Chaar, Hatem and Auguin, Daniel and Mom, Robin and Label, Philippe and Venisse, Jean-Stephane | ||||||
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Title: | Fungal X-Intrinsic Protein Aquaporin from Trichoderma atroviride: Structural and Functional Considerations | ||||||
Series Name/Journal: | Biomolecules | ||||||
Year of publishing : | 2021 | ||||||
Volume: | 11 | ||||||
Article number: | 338 | ||||||
Number of Pages: | 27 | ||||||
Publisher: | MDPI | ||||||
ISSN: | 2218-273X | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology | ||||||
Keywords: | aquaporin, uncharacterized X-Intrinsic proteins, Trichoderma atroviride, 3D modeling, chlamydospores, pentose phosphate pathway, stress responses | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-111126 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111126 | ||||||
Additional ID: |
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ID Code: | 22815 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Dept. of Forest Mycology and Plant Pathology (S) > Dept. of Forest Mycology and Plant Pathology | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 22 Mar 2021 09:03 | ||||||
Metadata Last Modified: | 22 Mar 2021 09:11 |
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