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Sperm chromatin integrity and DNA methylation in Norwegian Red bulls of contrasting fertility

Narud, Birgitte and Khezri, Abdolrahman and Zeremichael, Teklu Tewoldebrhan and Stenseth, Else-Berit and Heringstad, Bjorg and Johannisson, Anders and Morrell, Jane and Collas, Philippe and Myromslien, Froydis Deinboll and Kommisrud, Elisabeth (2021). Sperm chromatin integrity and DNA methylation in Norwegian Red bulls of contrasting fertility. Molecular Reproduction and Development. 88 , 187-200
[Research article]

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Abstract

In this study, the complexity of chromatin integrity was investigated in frozen-thawed semen samples from 37 sires with contrasting fertility, expressed as 56-day non-return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed and compared with previously published data for DNA fragmentation index (DFI) and high DNA stainability (HDS). In addition, in vitro embryo development and sperm DNA methylation were assessed using semen samples from 16 of these bulls. The percentages of DFI and HDS were negatively associated with NR56 and cleavage rate and positively associated with sperm protamine deficiency (p < 0.05). Significant differences in cleavage and blastocyst rates were observed between bulls of high and low NR56. However, once fertilization occurred, further development into blastocysts was not associated with NR56. The differential methylation analysis showed that spermatozoa from bulls of low NR56 were hypermethylated compared to bulls of high NR56. Pathway analysis showed that genes annotated to differentially methylated cytosines could participate in different biological pathways and have important biological roles related to bull fertility. In conclusion, sperm cells from Norwegian Red bulls of inferior fertility have less compact chromatin structure, higher levels of DNA damage, and are hypermethylated compared with bulls of superior fertility.

Authors/Creators:Narud, Birgitte and Khezri, Abdolrahman and Zeremichael, Teklu Tewoldebrhan and Stenseth, Else-Berit and Heringstad, Bjorg and Johannisson, Anders and Morrell, Jane and Collas, Philippe and Myromslien, Froydis Deinboll and Kommisrud, Elisabeth
Title:Sperm chromatin integrity and DNA methylation in Norwegian Red bulls of contrasting fertility
Series Name/Journal:Molecular Reproduction and Development
Year of publishing :2021
Volume:88
Page range:187-200
Number of Pages:14
Publisher:WILEY
ISSN:1040-452X
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:bull sperm, chromatin integrity, in vitro fertilization, non‐return rate, sperm DNA methylation
URN:NBN:urn:nbn:se:slu:epsilon-p-111111
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111111
Additional ID:
Type of IDID
DOI10.1002/mrd.23461
Web of Science (WoS)000621750200001
ID Code:22834
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:25 Mar 2021 09:23
Metadata Last Modified:25 Mar 2021 09:31

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