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Microbial controls on net production of nitrous oxide in a denitrifying woodchip bioreactor

Nordstrom, Albin and Hellman, Maria and Hallin, Sara and Herbert, Roger B. (2021). Microbial controls on net production of nitrous oxide in a denitrifying woodchip bioreactor. Journal of Environmental Quality. 50 , 228-240
[Journal article]

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Abstract

Denitrifying woodchip bioreactors are potential low-cost technologies for the removal of nitrate (NO3-) in water through denitrification. However, if environmental conditions do not support microbial communities performing complete denitrification, other N transformation processes will occur, resulting in the export of nitrite (NO2-), nitrous oxide (N2O), or ammonium (NH4+). To identify the factors controlling the relative accumulation of NO2-, N2O, and/or NH4+ in denitrifying woodchip bioreactors, porewater samples were collected over two operational years from a denitrifying woodchip bioreactor designed for removing NO3- from mine water. Woodchip samples were collected at the end of the operational period. Changes in the abundances of functional genes involved in denitrification, N2O reduction, and dissimilatory NO3- reduction to NH4+ were correlated with porewater chemistry and temperature. Temporal changes in the abundance of the denitrification gene nirS were significantly correlated with increases in porewater N2O concentrations and indicated the preferential selection of incomplete denitrifying pathways ending with N2O. Temperature and the total organic carbon/NO3- ratio were strongly correlated with NH4+ concentrations and inversely correlated with the ratio between denitrification genes and the genes indicative of ammonification (sigma nir/nrfA), suggesting an environmental control on NO3- transformations. Overall, our results for a denitrifying woodchip bioreactor operated at hydraulic residence times of 1.0-2.6 d demonstrate the temporal development in the microbial community and indicate an increased potential for N2O emissions with time from the denitrifying woodchip bioreactor.

Authors/Creators:Nordstrom, Albin and Hellman, Maria and Hallin, Sara and Herbert, Roger B.
Title:Microbial controls on net production of nitrous oxide in a denitrifying woodchip bioreactor
Series Name/Journal:Journal of Environmental Quality
Year of publishing :2021
Volume:50
Page range:228-240
Number of Pages:13
Publisher:WILEY
ISSN:0047-2425
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 208 Environmental Biotechnology > Water Treatment
URN:NBN:urn:nbn:se:slu:epsilon-p-111227
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111227
Additional ID:
Type of IDID
DOI10.1002/jeq2.20181
Web of Science (WoS)000617480400019
ID Code:22859
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:30 Mar 2021 07:03
Metadata Last Modified:30 Mar 2021 07:11

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