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Antifungal properties of dairy propionibacteria

Lind, Helena (2010). Antifungal properties of dairy propionibacteria. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2010:44
ISBN 978-91-576-7457-9
[Doctoral thesis]

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Today global agriculture is facing major challenges. The world's population is growing rapidly and we need to meet the increasing need for food with new strategies, including environmentally sustainable practices. Production must be enhanced with optimised resource utilisation and improved storage and distribution techniques. In addition, consumer demands for a reduction in artificial preservatives require innovative research thinking. The use of microorganisms as biopreservatives has many advantages. They often originate from environments similar to those where they will be used, making their presence natural. Bacteria such as lactic acid bacteria and dairy propionibacteria produce weak organic acids with good preserving capacity and several other antimicrobial compounds. Dairy propionibacteria possess additional advantageous features such as high production of vitamin b₁₂ and folic acid. Some strains also produce exopolysaccharides, useful for texture enhancement in dairy products. This thesis focuses on the antifungal properties of dairy propionibacteria. An important part of their antifungal capacity is due to the production of organic acids. The effect of these acids at different ph and different concentrations against eight spoilage fungi was investigated. A ph-dependent growth inhibition effect was detected, with Penicillium roqueforti being the most sensitive fungal species. In addition, dairy propionibacteria produce a number of other compounds with antifungal activity. Identification of 3-phenyl lactic acid and several antifungal peptides present in the growth medium was made after separation with a solid phase extraction column and hplc. The antifungal activity was monitored by screening against Aspergillus fumigatus. Strong enhancement of the antifungal activity from dairy propionibacteria in combination with glycerol was demonstrated. Addition of 500 mm glycerol to the upper layer in an overlay assay reduced the growth of some spoilage fungi completely. An antifungal strain of Propionibacterium jensenii was combined with Lactobacillus fermentum in a biopreserving culture used in moist grain preservation. The addition of glycerol to the system enhanced production of propionic acid in some cases, and an earlier decline in ph was detected. However, this combination of bacteria was not able to reduce the growth of P. roqueforti.

Authors/Creators:Lind, Helena
Title:Antifungal properties of dairy propionibacteria
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2010
Number of Pages:51
ALLI. Helena Lind, Hans Jonsson and Johan Schnürer (2005). Antifungal effect of dairy propionibacteria – contribution of organic acids. International Journal of Food Microbiology 98, 157-165. II. Helena Lind, Jörgen Sjögren, Suresh Gohil, Lennart Kenne, Johan Schnürer and Anders Broberg (2007). Antifungal compounds from cultures of dairy propionibacteria type strains. FEMS Microbiology Letters 271, 310-315. III. Helena Lind, Anders Broberg, Karin Jacobsson, Hans Jonsson and Johan Schnürer. Glycerol enhances the antifungal activity of dairy propionibacteria (submitted). IV. Helena Lind, Malin Larsson, Hans Jonsson, Johan Schnürer and Karin Jacobsson. The use of Propionibacterium jensenii and lactic acid bacteria for the preservation of moist grain in the presence of glycerol (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7457-9
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:propionibacterium, propionic acid bacteria, lactic acid bacteria, organic acids, antifungal properties, glycerol, preservation
Keywords:dairy propionibacteria, organic acid, antifungal, glycerol, biopreservation
Permanent URL:
ID Code:2287
Department:(NL, NJ) > Dept. of Microbiology (until 161231)
Deposited By: Helena Lind
Deposited On:07 May 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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