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Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts

Chmielarz, Mikolaj and Blomqvist, Johanna and Sampels, Sabine and Sandgren, Mats and Passoth, Volkmar (2021). Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts. Biotechnology for Biofuels. 14 , 65
[Research article]

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Abstract

Background Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low-value side-products of biodiesel transesterification and pulp-and paper industry or lignocellulosic ethanol production, respectively, which can be converted to microbial lipids by oleaginous yeasts. This study aimed to test the ability of oleaginous yeasts to utilise CG and HH and mixtures of them as carbon source. Results Eleven out of 27 tested strains of oleaginous yeast species were able to grow in plate tests on CG as sole carbon source. Among them, only one ascomycetous strain, belonging to Lipomyces starkeyi, was identified, the other 10 strains were Rhodotorula spec. When yeasts were cultivated in mixed CG/ HH medium, we observed an activation of glycerol conversion in the Rhodotorula strains, but not in L. starkeyi. Two strains-Rhodotorula toruloides CBS 14 and Rhodotorula glutinis CBS 3044 were further tested in controlled fermentations in bioreactors in different mixtures of CG and HH. The highest measured average biomass and lipid concentration were achieved with R. toruloides in 10% HH medium mixed with 55 g/L CG-19.4 g/L and 10.6 g/L, respectively, with a lipid yield of 0.25 g lipids per consumed g of carbon source. Fatty acid composition was similar to other R. toruloides strains and comparable to that of vegetable oils. Conclusions There were big strain differences in the ability to convert CG to lipids, as only few of the tested strains were able to grow. Lipid production rates and yields showed that mixing GC and HH have a stimulating effect on lipid accumulation in R. toruloides and R. glutinis resulting in shortened fermentation time to reach maximum lipid concentration, which provides a new perspective on converting these low-value compounds to microbial lipids.

Authors/Creators:Chmielarz, Mikolaj and Blomqvist, Johanna and Sampels, Sabine and Sandgren, Mats and Passoth, Volkmar
Title:Microbial lipid production from crude glycerol and hemicellulosic hydrolysate with oleaginous yeasts
Series Name/Journal:Biotechnology for Biofuels
Year of publishing :2021
Volume:14
Article number:65
Number of Pages:11
Publisher:BMC
ISSN:1754-6834
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Biocatalysis and Enzyme Technology
Keywords:Oleaginous yeast, Hemicelluloses, Crude glycerol, Lipids, R, torouloides
URN:NBN:urn:nbn:se:slu:epsilon-p-111404
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111404
Additional ID:
Type of IDID
DOI10.1186/s13068-021-01916-y
Web of Science (WoS)000628217500001
ID Code:23258
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:13 Apr 2021 15:03
Metadata Last Modified:13 Apr 2021 15:11

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