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Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential

Rocha, Elerson Matos and Marinotti, Osvaldo and Serrao, Deidre Machado and Correa, Laura Viana and Katak, Ricardo de Melo and Campos de Oliveira, Juan and Muniz, Veranilce Alves and de Oliveira, Marta Rodrigues and Ferreira, Joaquim and Pessoa, Marcos Cezar Fernandes and da Mota, Adolfo Jose and Roque, Rosemary Aparecida and Onorati, Piero and Souza-Neto, Jayme A. and Terenius, Olle and Tadei, Wanderli Pedro (2021). Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential. Malaria Journal. 20 , 40
[Research article]

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Abstract

Background Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.

Authors/Creators:Rocha, Elerson Matos and Marinotti, Osvaldo and Serrao, Deidre Machado and Correa, Laura Viana and Katak, Ricardo de Melo and Campos de Oliveira, Juan and Muniz, Veranilce Alves and de Oliveira, Marta Rodrigues and Ferreira, Joaquim and Pessoa, Marcos Cezar Fernandes and da Mota, Adolfo Jose and Roque, Rosemary Aparecida and Onorati, Piero and Souza-Neto, Jayme A. and Terenius, Olle and Tadei, Wanderli Pedro
Title:Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential
Series Name/Journal:Malaria Journal
Year of publishing :2021
Volume:20
Article number:40
Number of Pages:9
ISSN:1475-2875
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:Mosquito, Malaria, Microbiota, Vector-borne disease, Amazon forest
URN:NBN:urn:nbn:se:slu:epsilon-p-109965
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109965
Additional ID:
Type of IDID
DOI10.1186/s12936-020-03574-1
Web of Science (WoS)000609496200001
ID Code:23270
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:15 Apr 2021 06:43
Metadata Last Modified:03 Aug 2021 14:01

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