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Lipid production from lignocellulosic material by oleaginous yeasts

Brandenburg, Jule (2021). Lipid production from lignocellulosic material by oleaginous yeasts. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880
ISBN 978-91-7760-738-0
eISBN 978-91-7760-739-7
[Doctoral thesis]

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Abstract

Oleaginous yeasts are a potential lipid source for production of fuels, chemicals and food or feed and use of lignocellulosic biomass as feedstock is considered a sustainable approach. Pre-treatment of lignocellulose is required to release the major carbon sources, glucose, xylose and other sugars for lipid production by oleaginous yeasts, but also releases inhibitory compounds. Aim of this thesis was to examine the potential for producing lipids from different lignocellulosic substrates using oleaginous yeasts and to develop analytical methods for monitoring the kinetics of lipid accumulation, as a basis for further investigations of physiological differences in oleaginous yeasts on different substrates. Investigations of 29 different oleaginous yeast strains revealed considerable differences in xylose utilisation capacity, even among very closely related strains. Some strains were very efficient in accumulating lipids from all carbon sources in lignocellulose hydrolysate, others showed no or only weak growth on xylose and in one case intracellular lipid degradation during consumption of xylose was observed. Further investigation demonstrated that it is possible to combine furfural production from hemicellulose and microbial lipid or ethanol production from the cellulose fraction of wheat straw material. An investigation of lipid production from birch wood hemicellulose containing high amounts of xylose and acetic acid revealed that by establishing a pH-regulated feeding strategy, acetic acid could be utilised as an additional carbon source and no growth inhibition was observed. Target parameters when studying lipid-accumulating yeasts are intracellular lipid content and lipid profile. However, classical extraction-based analytical methods are time- and workintensive. Therefore, a non-invasive method based on high-throughput Fourier transform infrared (FTIR) spectroscopy was established. Overall, large diversity among oleaginous yeasts was revealed, especially when converting xylose. Promising strains for lipid production from different substrates were identified, providing a baseline for further studies on the physiology of oleaginous yeasts and on biotechnological production of microbial lipids.

Authors/Creators:Brandenburg, Jule
Title:Lipid production from lignocellulosic material by oleaginous yeasts
Series Name/Journal:Acta Universitatis Agriculturae Sueciae
Year of publishing :2021
Number:2021:30
Number of Pages:81
Publisher:Department of Molecular Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-738-0
ISBN for electronic version:978-91-7760-739-7
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Bioenergy
Keywords:microbial lipids, FTIR, lipid extraction, hydrolysate, biorefinery
URN:NBN:urn:nbn:se:slu:epsilon-p-111449
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111449
ID Code:23277
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:15 Apr 2021 12:23
Metadata Last Modified:21 Apr 2021 12:48

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