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Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Rimet, Frédéric and Aylagas, Eva and Borja, Angel and Bouchez, Agnes and Canino, Alexis and Chauvin, Christian and Chonova, Teofana and Ciampor, Fedor and Costa, Filipe O. and Ferrari, Benoit J. D. and Gastineau, Romain and Goulon, Chloé and Gugger, Muriel and Holzmann, Maria and Jahn, Regine and Kahlert, Maria and Kusber, Wolf-Henning and Laplace-Treyture, Christophe and Leese, Florian and Leliaert, Frederik and Mann, David G. and Marchand, Frédéric and Méléder, Vona and Pawlowski, Jan and Rasconi, Serena and Rivera, Sinziana and Rougerie, Rodolphe and Schweizer, Magali and Trobajo, Rosa and Vasselon, Valentin and Vivien, Régis and Weigand, Alexander M. and Witkowski, Andrzej and Zimmermann, Jonas and Ekrem, Torbjorn (2021). Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life. Metabarcoding and Metagenomics. 5 , e58056 , 17-33
[Research article]

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Abstract

DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.

Authors/Creators:Rimet, Frédéric and Aylagas, Eva and Borja, Angel and Bouchez, Agnes and Canino, Alexis and Chauvin, Christian and Chonova, Teofana and Ciampor, Fedor and Costa, Filipe O. and Ferrari, Benoit J. D. and Gastineau, Romain and Goulon, Chloé and Gugger, Muriel and Holzmann, Maria and Jahn, Regine and Kahlert, Maria and Kusber, Wolf-Henning and Laplace-Treyture, Christophe and Leese, Florian and Leliaert, Frederik and Mann, David G. and Marchand, Frédéric and Méléder, Vona and Pawlowski, Jan and Rasconi, Serena and Rivera, Sinziana and Rougerie, Rodolphe and Schweizer, Magali and Trobajo, Rosa and Vasselon, Valentin and Vivien, Régis and Weigand, Alexander M. and Witkowski, Andrzej and Zimmermann, Jonas and Ekrem, Torbjorn
Title:Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
Series Name/Journal:Metabarcoding and Metagenomics
Year of publishing :2021
Volume:5
Article number:e58056
Number of Pages:17
ISSN:2534-9708
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
URN:NBN:urn:nbn:se:slu:epsilon-p-111140
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111140
Additional ID:
Type of IDID
DOI10.3897/mbmg.5.58056
ID Code:23288
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:19 Apr 2021 07:43
Metadata Last Modified:19 Apr 2021 07:51

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