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Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress

Malabarba, Jaiana and Windels, David and Xu, Wenjia and Verdier, Jerome (2021). Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress. Genes. 12 , 457
[Research article]

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Abstract

Seed development needs the coordination of multiple molecular mechanisms to promote correct tissue development, seed filling, and the acquisition of germination capacity, desiccation tolerance, longevity, and dormancy. Heat stress can negatively impact these processes and upon the increase of global mean temperatures, global food security is threatened. Here, we explored the impact of heat stress on seed physiology, morphology, gene expression, and methylation on three stages of seed development. Notably, Arabidopsis Col-0 plants under heat stress presented a decrease in germination capacity as well as a decrease in longevity. We observed that upon mild stress, gene expression and DNA methylation were moderately affected. Nevertheless, upon severe heat stress during seed development, gene expression was intensively modified, promoting heat stress response mechanisms including the activation of the ABA pathway. By analyzing candidate epigenetic markers using the mutants' physiological assays, we observed that the lack of DNA demethylation by the ROS1 gene impaired seed germination by affecting germination-related gene expression. On the other hand, we also observed that upon severe stress, a large proportion of differentially methylated regions (DMRs) were located in the promoters and gene sequences of germination-related genes. To conclude, our results indicate that DNA (de)methylation could be a key regulatory process to ensure proper seed germination of seeds produced under heat stress.

Authors/Creators:Malabarba, Jaiana and Windels, David and Xu, Wenjia and Verdier, Jerome
Title:Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress
Series Name/Journal:Genes
Year of publishing :2021
Volume:12
Article number:457
Number of Pages:21
Publisher:MDPI
ISSN:2073-4425
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:heat stress, seed, DNA methylation, seed development, seed germination
URN:NBN:urn:nbn:se:slu:epsilon-p-111477
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111477
Additional ID:
Type of IDID
DOI10.3390/genes12030457
Web of Science (WoS)000633656400001
ID Code:23297
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:19 Apr 2021 13:58
Metadata Last Modified:19 Apr 2021 14:01

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