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Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96

Papin (nee Richter), Johanna and Stuhtmann, Gesa and Martinsson, Gunilla and Sieme, Harald and Lundeheim, Nils and Ntallaris, Theodoros and Morrell, Jane (2021). Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96. Journal of Equine Veterinary Science. 98 , 103367
[Research article]

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Abstract

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage sperm chromatin. Therefore, sperm quality was compared in samples following single-layer centrifugation (SLC) or sperm washing and controls (extension only) in two extenders, INRA82 and INRA96. Ejaculates from six stallions were split among six treatments: SLC, sperm washing, and controls, in INRA82 and INRA96. Sperm motility and acrosome status were evaluated at 0, 24, 48, 72 and 96 hours; morphology at 0, 24, 48, 72 hours and chromatin integrity at 0 and 96 hours, with storage at 6 degrees C. Sperm samples in INRA96 had better motility, acrosome status, and normal morphology than samples in INRA82. The SLC samples had higher motility and fewer reacted acrosomes than controls, and lower fragmented chromatin than washed samples. Fewer spermatozoa with tail defects were observed after SLC than after sperm washing; spermatozoa washed in INRA82 had fewer tail defects than those washed in INRA96. In conclusion, sperm quality (except for morphology) was better in INRA96 than in INRA82 and was better in SLC samples than in washed samples or controls. The SLC method is a useful adjunct to stallion sperm preparation, especially for storage before artificial insemination. (C) 2020 The Author(s). Published by Elsevier Inc.

Authors/Creators:Papin (nee Richter), Johanna and Stuhtmann, Gesa and Martinsson, Gunilla and Sieme, Harald and Lundeheim, Nils and Ntallaris, Theodoros and Morrell, Jane
Title:Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96
Series Name/Journal:Journal of Equine Veterinary Science
Year of publishing :2021
Volume:98
Article number:103367
Number of Pages:8
ISSN:0737-0806
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Colloid centrifugation, Stallion semen, Sperm motility, Chromatin integrity, Acrosome status
URN:NBN:urn:nbn:se:slu:epsilon-p-111174
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111174
Additional ID:
Type of IDID
DOI10.1016/j.jevs.2020.103367
Web of Science (WoS)000626376600006
ID Code:23308
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Breeding and Genetics
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:20 Apr 2021 09:23
Metadata Last Modified:20 Apr 2021 09:31

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