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Metabolic regulation in developing barley seeds

novel insights from transcriptome analyses

Mangelsen, Elke (2010). Metabolic regulation in developing barley seeds. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2010:50
ISBN 978-91-576-7463-0
[Doctoral thesis]

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Abstract

The barley (Hordeum vulgare) seed, termed caryopsis, is of high importance for feed and food industry. In addition, it provides an excellent model to study the development and physiology of starch-storing cereal seeds. Gene expression in caryopses, like all plant organs, is to a large extent regulated by the cellular sugar status. As part of the sugar signalling cascade, we have previously identified the sucrose-inducible barley WRKY transcription factor SUSIBA2 which activates an isoamylase gene involved in starch biosynthesis. Subsequently, the aims of this thesis work were i) to clarify the phylogenetic relationship of SUSIBA2 with other WRKY genes and ii) to investigate the effects of diurnal changes and heat stress on metabolite levels and global gene expression in order to identify new potentially sugar status-dependent processes in developing barley caryopses. A total number of 45 barley WRKY gene family members were identified. Based on comparative phylogenetic analyses with genes from Arabidopsis (A. thaliana) and rice (Oryza sativa), barley WRKY genes putatively involved in development and stress response were assigned. In an attempt to non-invasively study the effect of altered sugar levels, we applied the 22K Barley1 GeneChip microarray to monitor the diurnal changes of global gene expression in barley caryopses. Among 2,091 diurnally regulated genes, almost 900 were highly correlated or anti-correlated to sugar levels, and, thus, potentially sugar status-regulated. Investigations of the shortterm (0.5h, 3h and 6h) exposure to heat identified stress response mechanisms and revealed a global repression of carbon metabolism-related genes after as short as 3h of heat stress. Summarized, this thesis provides the barley research community with a repository of genes involved in metabolic regulation of barley caryopsis development during its adaptation to diurnal changes and heat stress. Candidate genes were identified which merit further investigation of their functions in order to understand and, thereon, modify sugar signalling and carbon metabolism in the barley caryopsis as a model for cereal seeds.

Authors/Creators:Mangelsen, Elke
Title:Metabolic regulation in developing barley seeds
Subtitle:novel insights from transcriptome analyses
Year of publishing :2010
Volume:2010:50
Number of Pages:56
Papers/manuscripts:
NumberReferences
ALLI. Mangelsen, E., Kilian, J., Berendzen, K.W., Kolukisaoglu, H.Ü., Harter, K., Jansson, C., Wanke, D. (2008). Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots. BMC Genomics 9, 194. II. Mangelsen, E., Wanke, D., Kilian, J., Sundberg, E., Harter, K., Jansson, C. (2010). Significance of Light, Sugar and Amino Acid Supply for Diurnal Gene Regulation in Developing Barley Caryopses. Plant Physiology 153(1), 14-33. III. Mangelsen, E., Kilian J., Harter, K., Jansson, C., Wanke, D., Sundberg, E. (2010). Global gene expression analysis of high-temperature stress in developing barley caryopses: Early stress response mechanisms and effects on storage compound biosynthesis. Accepted in Molecular Plant.
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7463-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:hordeum vulgare, seeds, caryopses, biological development, gene expression, transcription factors, biological rhythms, abiotic stress, heat stress, sucrose, starch
Keywords:transcriptome, cereal, seed, storage, diurnal, abiotic stress, heat shock, WRKY, sucrose, starch
URN:NBN:urn:nbn:se:slu:epsilon-3085
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3085
ID Code:2333
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Elke Mangelsen
Deposited On:25 Aug 2010 00:00
Metadata Last Modified:02 Dec 2014 10:17

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