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Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus

Berenguer, Eduardo and Minina, Elena A. and Carneros, Elena and Bárány, Ivett and Bozhkov, Peter and Testillano, Pilar S. (2020). Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus. Plant and Cell Physiology. 61 , 2097-2110
[Research article]

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Abstract

Microspore embryogenesis is a biotechnological process that allows us to rapidly obtain doubled-haploid plants for breeding programs. The process is initiated by the application of stress treatment, which reprograms microspores to embark on embryonic development. Typically, a part of the microspores undergoes cell death that reduces the efficiency of the process. Metacaspases (MCAs), a phylogenetically broad group of cysteine proteases, and autophagy, the major catabolic process in eukaryotes, are critical regulators of the balance between cell death and survival in various organisms. In this study, we analyzed the role of MCAs and autophagy in cell death during stress-induced microspore embryogenesis in Brassica napus. We demonstrate that this cell death is accompanied by the transcriptional upregulation of three BnMCA genes (BnMCA-Ia, BnMCA-IIa and BnMCA-IIi), an increase in MCA proteolytic activity and the activation of autophagy. Accordingly, inhibition of autophagy and MCA activity, either individually or in combination, suppressed cell death and increased the number of proembryos, indicating that both components play a pro-cell death role and account for decreased efficiency of early embryonic development. Therefore, MCAs and/or autophagy can be used as new biotechnological targets to improve in vitro embryogenesis in Brassica species and doubled-haploid plant production in crop breeding and propagation programs.

Authors/Creators:Berenguer, Eduardo and Minina, Elena A. and Carneros, Elena and Bárány, Ivett and Bozhkov, Peter and Testillano, Pilar S.
Title:Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus
Series Name/Journal:Plant and Cell Physiology
Year of publishing :2020
Volume:61
Page range:2097-2110
Number of Pages:14
ISSN:0032-0781
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:autophagy, metacaspase, microspore embryogenesis, programmed cell death, rapeseed, stress
URN:NBN:urn:nbn:se:slu:epsilon-p-109921
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-109921
Additional ID:
Type of IDID
DOI10.1093/pcp/pcaa128
Web of Science (WoS)000627419900010
ID Code:23383
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:29 Apr 2021 15:03
Metadata Last Modified:29 Apr 2021 15:11

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