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Function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis

Vaughan-Hirsch, John and Tallerday, Emily J. and Burr, Christian A. and Hodgens, Charlie and Boeshore, Samantha L. and Beaver, Kevin and Melling, Allison and Sari, Kartika and Kerr, Ian D. and Simura, Jan and Ljung, Karin and Xu, Dawei and Liang, Wanqi and Bhosale, Rahul and Schaller, G. Eric and Bishopp, Anthony and Kieber, Joseph J. (2021). Function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis. Plant Journal. 106 , 159-173
[Research article]

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Abstract

The phytohormone cytokinin plays a significant role in nearly all aspects of plant growth and development. Cytokinin signaling has primarily been studied in the dicot model Arabidopsis, with relatively little work done in monocots, which include rice (Oryza sativa) and other cereals of agronomic importance. The cytokinin signaling pathway is a phosphorelay comprised of the histidine kinase receptors, the authentic histidine phosphotransfer proteins (AHPs) and type-B response regulators (RRs). Two negative regulators of cytokinin signaling have been identified: the type-A RRs, which are cytokinin primary response genes, and the pseudo histidine phosphotransfer proteins (PHPs), which lack the His residue required for phosphorelay. Here, we describe the role of the rice PHP genes. Phylogenic analysis indicates that the PHPs are generally first found in the genomes of gymnosperms and that they arose independently in monocots and dicots. Consistent with this, the three rice PHPs fail to complement an Arabidopsis php mutant (aphp1/ahp6). Disruption of the three rice PHPs results in a molecular phenotype consistent with these elements acting as negative regulators of cytokinin signaling, including the induction of a number of type-A RR and cytokinin oxidase genes. The triple php mutant affects multiple aspects of rice growth and development, including shoot morphology, panicle architecture, and seed fill. In contrast to Arabidopsis, disruption of the rice PHPs does not affect root vascular patterning, suggesting that while many aspects of key signaling networks are conserved between monocots and dicots, the roles of at least some cytokinin signaling elements are distinct.

Authors/Creators:Vaughan-Hirsch, John and Tallerday, Emily J. and Burr, Christian A. and Hodgens, Charlie and Boeshore, Samantha L. and Beaver, Kevin and Melling, Allison and Sari, Kartika and Kerr, Ian D. and Simura, Jan and Ljung, Karin and Xu, Dawei and Liang, Wanqi and Bhosale, Rahul and Schaller, G. Eric and Bishopp, Anthony and Kieber, Joseph J.
Title:Function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis
Series Name/Journal:Plant Journal
Year of publishing :2021
Volume:106
Page range:159-173
Number of Pages:15
Publisher:WILEY
ISSN:0960-7412
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Oryza sativa, plant hormones, cytokinin, two‐component signaling
URN:NBN:urn:nbn:se:slu:epsilon-p-110930
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-110930
Additional ID:
Type of IDID
DOI10.1111/tpj.15156
Web of Science (WoS)000615949300001
ID Code:23408
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:04 May 2021 08:23
Metadata Last Modified:04 May 2021 08:31

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