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Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3

Bobkov, Yuriy V. and Walker, William B. and Cattaneo, Alberto Maria (2021). Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3. Scientific Reports. 11 , 3893
[Research article]

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Abstract

Amino acid substitutions within the conserved polypeptide sequence of the insect olfactory receptor co-receptor (Orco) have been demonstrated to influence its pharmacological properties. By sequence analysis and phylogenetic investigation, in the Lepidopteran subgroup Ditrysia we identified a fixed substitution in the intracellular loop-3 (ICL-3) of a conserved histidine to glutamine. By means of HEK293 cells as a heterologous system, we functionally expressed Orco from the Ditrysian model Cydia pomonella (CpomOrco) and compared its functional properties with a site-directed mutagenized version where this ICL-3-glutamine was reverted to histidine (CpomOrco(Q417H)). The mutagenized CpomOrco(Q417H) displayed decreased responsiveness to VUAA1 and reduced response efficacy to an odorant agonist was observed, when co-transfected with the respective OR subunit. Evidence of reduced responsiveness and sensitivity to ligands for the mutagenized Orco suggest the fixed glutamine substitution to be optimized for functionality of the cation channel within Ditrysia. In addition, contrary to the wild type, the mutagenized CpomOrco(Q417H) preserved characteristics of VUAA-binding when physiologic conditions turned to acidic. Taken together, our findings provide further evidence of the importance of ICL-3 in forming basic functional properties of insect Orco- and Orco/OR-channels, and suggest involvement of ICL-3 in the potential functional adaptation of Ditrysian Orcos to acidified extra-/intracellular environment.

Authors/Creators:Bobkov, Yuriy V. and Walker, William B. and Cattaneo, Alberto Maria
Title:Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3
Series Name/Journal:Scientific Reports
Year of publishing :2021
Volume:11
Article number:3893
Number of Pages:16
Publisher:NATURE RESEARCH
ISSN:2045-2322
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-111720
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111720
Additional ID:
Type of IDID
DOI10.1038/s41598-021-83024-3
Web of Science (WoS)000626727200044
ID Code:23432
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:06 May 2021 13:03
Metadata Last Modified:06 May 2021 13:11

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