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Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes

Rissanen, Antti J. and Saarela, Taija and Jantti, Helena and Buck, Moritz and Peura, Sari and Aalto, Sanni L. and Ojala, Anne and Pumpanen, Jukka and Tiirola, Marja and Elvert, Marcus and Nykanen, Hannu (2021). Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes. FEMS Microbiology Ecology. 97 , fiaa252
[Research article]

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Abstract

The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajarvi and Lake Lovojarvi. Furthermore, metagenomic analyses were performed to assess the genomic characteristics of methanotrophs in Lovojarvi and the previously studied Lake Alinen Mustajarvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived, especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in clusters of orthologous groups (COG) categories L (replication, recombination and repair) and S (function unknown) were relatively high in metagenome-assembled genomes representing Methylococcales clearly thriving below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. By contrast, genes in COG category N (cell motility) were relatively high in metagenome-assembled genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface.

Authors/Creators:Rissanen, Antti J. and Saarela, Taija and Jantti, Helena and Buck, Moritz and Peura, Sari and Aalto, Sanni L. and Ojala, Anne and Pumpanen, Jukka and Tiirola, Marja and Elvert, Marcus and Nykanen, Hannu
Title:Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes
Series Name/Journal:FEMS Microbiology Ecology
Year of publishing :2021
Volume:97
Article number:fiaa252
Number of Pages:16
Publisher:OXFORD UNIV PRESS
ISSN:0168-6496
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:methanotroph, Methylococcales, vertical structuring, 16S rRNA, metagenomics, genetic potential
URN:NBN:urn:nbn:se:slu:epsilon-p-111764
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111764
Additional ID:
Type of IDID
DOI10.1093/femsec/fiaa252
Web of Science (WoS)000637052400009
ID Code:23474
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:11 May 2021 14:43
Metadata Last Modified:11 May 2021 14:51

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