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Protein Nanofibrils and Their Hydrogel Formation with Metal Ions

Ye, Xinchen and Capezza, Antonio J. and Xiao, Xiong and Lendel, Christofer and Hedenqvist, Mikael S. and Kessler, Vadim and Olsson, Richard T. (2021). Protein Nanofibrils and Their Hydrogel Formation with Metal Ions. ACS Nano. 15 , 5341-5354
[Research article]

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Abstract

Protein nanofibrils (PNFs) have been prepared by whey protein fibrillation at low pH and in the presence of different metal ions. The effect of the metal ions was systematically studied both in terms of PNF suspension gelation behavior and fibrillation kinetics. A high valence state and a small ionic radius (e.g., Sn4+) of the metal ion resulted in the formation of hydrogels already at a metal ion concentration of 30 mM, whereas an intermediate valence state and larger ionic radius (Co2+, Ni2+, Al3+) resulted in the hydrogel formation occurring at 60 mM. A concentration of 120 mM of Na+ was needed to form a PNF hydrogel, while lower concentrations showed liquid behaviors similar to the reference PNF solution where no metal ions had been introduced. The hydrogel mechanics were investigated at steady-state conditions after 24 h of incubation/gelation, revealing that more acidic (smaller and more charged) metal ions induced ca. 2 orders of magnitude higher storage modulus as compared to the less acidic metal ions (with smaller charge and larger radius) for the same concentration of metal ions. The viscoelastic nature of the hydrogels was attributed to the ability of the metal ions to coordinate water molecules in the vicinity of the PNFs. The presence of metal ions in the solutions during the growth of the PNFs typically resulted in curved fibrils, whereas an upper limit of the concentration existed when oxides/hydroxides were formed, and the hydrogels lost their gel properties due to phase separation. Thioflavin T (ThT) fluorescence was used to determine the rate of the fibrillation to form 50% of the total PNFs (t(1/2)), which decreased from 2.3 to ca. 0.5 h depending on the specific metal ions added.

Authors/Creators:Ye, Xinchen and Capezza, Antonio J. and Xiao, Xiong and Lendel, Christofer and Hedenqvist, Mikael S. and Kessler, Vadim and Olsson, Richard T.
Title:Protein Nanofibrils and Their Hydrogel Formation with Metal Ions
Series Name/Journal:ACS Nano
Year of publishing :2021
Volume:15
Page range:5341-5354
Number of Pages:14
Publisher:AMER CHEMICAL SOC
ISSN:1936-0851
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Polymer Chemistry
Keywords:protein nanofibrils, whey protein, hydrogels, metal ions, kinetics
URN:NBN:urn:nbn:se:slu:epsilon-p-111767
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111767
Additional ID:
Type of IDID
DOI10.1021/acsnano.0c10893
Web of Science (WoS)000634569100142
ID Code:23712
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:19 May 2021 10:43
Metadata Last Modified:19 May 2021 10:51

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