Home About Browse Search
Svenska


Fecal microbiota in children with juvenile idiopathic arthritis treated with methotrexate or etanercept

Oman, Anders and Dicksved, Johan and Engstrand, Lars and Berntson, Lillemor (2021). Fecal microbiota in children with juvenile idiopathic arthritis treated with methotrexate or etanercept. Pediatric Rheumatology. 19 , 55
[Research article]

[img] PDF
994kB

Abstract

Background Alterations in the composition of the fecal microbiota in children with juvenile idiopathic arthritis (JIA) have been observed in several studies, but it has not been determined whether the standard treatment for JIA changes the composition or function of the microbiota. The first-line disease-modifying anti-rheumatic drug for treatment of JIA is usually methotrexate, followed or supplemented by anti-tumor necrosis factor alpha drugs, such as etanercept. The aim of this study was to investigate the effects of methotrexate and etanercept treatments on the fecal microbiota and the fecal short-chain fatty acids (SCFAs) in children with JIA. Methods In this multicenter study, the composition of fecal microbiota from 45 treatment-naive children with JIA was compared with that from 29 children treated with methotrexate and 12 children treated with etanercept. We also made pairwise comparisons of 15 children sampled before and during methotrexate treatment and 7 children sampled before and during etanercept treatment. The microbiota was determined using sequencing amplicons from the V3 and V4 regions of the 16S rRNA gene. Alpha-diversity, community composition, and relative abundances of bacterial taxa were analyzed in all comparisons. Analyses of fecal SCFAs, using a high-performance liquid chromatograph, were performed for the pairwise comparisons. Results We did not find any significant differences in alpha-diversity or community composition of microbiota. However, principal coordinate analysis indicated a change in community composition in 7 of the 15 paired samples before and during methotrexate and 2 of the 7 paired samples before and during etanercept. Comparisons of the relative abundance of taxa revealed minor differences before and during treatment with methotrexate or etanercept, but they were not significant after correction for multiple analyses, and the unpaired and paired analyses did not show similar changes. There were no significant differences in levels of fecal SCFAs before and during treatment with methotrexate or etanercept. Conclusions Treatment with methotrexate or etanercept had minor, but no significant or consistent changes either on composition of microbiota or on levels of SCFAs, suggesting that these changes are not related to the therapeutic effects of methotrexate or etanercept.

Authors/Creators:Oman, Anders and Dicksved, Johan and Engstrand, Lars and Berntson, Lillemor
Title:Fecal microbiota in children with juvenile idiopathic arthritis treated with methotrexate or etanercept
Series Name/Journal:Pediatric Rheumatology
Year of publishing :2021
Volume:19
Article number:55
Number of Pages:10
Publisher:BMC
ISSN:1546-0096
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Rheumatology and Autoimmunity
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Microbiology in the medical area
Keywords:Arthritis, juvenile, Gastrointestinal microbiome, Short-chain fatty acids, Methotrexate, Etanercept
URN:NBN:urn:nbn:se:slu:epsilon-p-111917
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111917
Additional ID:
Type of IDID
DOI10.1186/s12969-021-00542-0
Web of Science (WoS)000644638600001
ID Code:23733
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:21 May 2021 11:23
Metadata Last Modified:21 May 2021 11:31

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits