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Induction of Plant Resistance in Tobacco (Nicotiana tabacum) against Tomato Spotted Wilt Orthotospovirus through Foliar Application of dsRNA

Konakalla, Nagacharan and Bag, Sudeep and Deraniyagala, Anushi Suwaneththiya and Culbreath, Albert K. and Pappu, Hanu R. (2021). Induction of Plant Resistance in Tobacco (Nicotiana tabacum) against Tomato Spotted Wilt Orthotospovirus through Foliar Application of dsRNA. Viruses. 13 , 662
[Research article]

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Abstract

Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) continues to be a constraint to peanut, pepper, tobacco, and tomato production in Georgia and elsewhere. TSWV is being managed by an integrated disease management strategy that includes a combination of cultural practices, vector management, and growing virus-resistant varieties where available. We used a non-transgenic strategy to induce RNA interference (RNAi)-mediated resistance in tobacco (Nicotiana tabacum) plants against TSWV. Double-stranded RNA (dsRNA) molecules for the NSs (silencing suppressor) and N (nucleoprotein) genes were produced by a two-step PCR approach followed by in vitro transcription. When topically applied to tobacco leaves, both molecules elicited a resistance response. Host response to the treatments was measured by determining the time to symptom expression, and the level of resistance by absolute quantification of the virus. We also show the systemic movement of dsRNA_N from the inoculated leaves to younger, non-inoculated leaves. Post-application, viral siRNAs were detected for up to nine days in inoculated leaves and up to six days in non-inoculated leaves. The topical application of dsRNAs to induce RNAi represents an environmentally safe and efficient way to manage TSWV in tobacco crops and could be applicable to other TSWV-susceptible crops.

Authors/Creators:Konakalla, Nagacharan and Bag, Sudeep and Deraniyagala, Anushi Suwaneththiya and Culbreath, Albert K. and Pappu, Hanu R.
Title:Induction of Plant Resistance in Tobacco (Nicotiana tabacum) against Tomato Spotted Wilt Orthotospovirus through Foliar Application of dsRNA
Series Name/Journal:Viruses
Year of publishing :2021
Volume:13
Article number:662
Number of Pages:13
Publisher:MDPI
ISSN:1999-4915
Language:English
Publication Type:Research article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:double-stranded RNA, tomato spotted wilt virus, N gene, NSs gene, viral suppressors of RNA silencing, RNA interference, vsiRNA, virus resistance
URN:NBN:urn:nbn:se:slu:epsilon-p-111924
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-111924
Additional ID:
Type of IDID
DOI10.3390/v13040662
Web of Science (WoS)000643831500001
ID Code:23739
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:21 May 2021 14:23
Metadata Last Modified:21 May 2021 14:31

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